doi: 10.1128/JVI.30.1.417-420.1979.
Identification of mouse mammary tumor virus-specific mRNA
- PMID: 225528
- PMCID: PMC353340
- DOI: 10.1128/JVI.30.1.417-420.1979
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Identification of mouse mammary tumor virus-specific mRNA
J Virol.
1979 Apr.
Abstract
Complementary DNA corresponding to the RNA genome of mouse mammary tumor virus was used to identify viral RNA contained in polysomes of a virus-producing mammary tumor cell line. Separation of polysomal mRNA by agarose gel electrophoresis, transfer of the RNA to diazobenzyloxymethyl paper, and hybridization with 32P-labeled mouse mammary tumor virus complementary DNA revealed three viral RNA size classes of 10, 8.8, and 4.4 kilobases in length, respectively.
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