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. 1979 Apr;30(1):417-20.
doi: 10.1128/JVI.30.1.417-420.1979.

Identification of mouse mammary tumor virus-specific mRNA

B Groner, N E Hynes, H Diggelmann

Identification of mouse mammary tumor virus-specific mRNA

B Groner et al. J Virol. 1979 Apr.

Abstract

Complementary DNA corresponding to the RNA genome of mouse mammary tumor virus was used to identify viral RNA contained in polysomes of a virus-producing mammary tumor cell line. Separation of polysomal mRNA by agarose gel electrophoresis, transfer of the RNA to diazobenzyloxymethyl paper, and hybridization with 32P-labeled mouse mammary tumor virus complementary DNA revealed three viral RNA size classes of 10, 8.8, and 4.4 kilobases in length, respectively.

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References

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