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. 2010;3(2):99-103.
doi: 10.3980/j.issn.2222-3959.2010.02.02. Epub 2010 Jun 18.

Effects of flavone on the oxidation-induced injury of retinal pigment epithelium cells

Pei Zhuang  1 , Yi Shen, George C Y Chiou
Affiliations

Effects of flavone on the oxidation-induced injury of retinal pigment epithelium cells

Pei Zhuang et al. Int J Ophthalmol. 2010.

Abstract

Aim: To investigate the effect of flavone on oxidation-induced injury in retinal pigment epithelium cells.

Methods: In in vivo studies, NaIO(3)-induced RPE degeneration in rat eyes was treated with 0.5% flavone eye drops 3 times a day for 1 week before and 4 weeks after NaIO(3) injection. At the end of 2 and 4 weeks, all rats were measured c-wave by electroretinogram (ERG). In in vitro studies, ARPE-19 cells were treated with hypoxia, H(2)O(2), NaN(3) and t-BHP to induce cell damages. MTT assay was used to measure the viable cells.

Results: The ERG c-wave results showed that flavone reversed NaIO(3)-induced injury at the end of 4 weeks. In vitro results showed flavone reversed the various oxidants-induced injuries in RPE cells.

Conclusion: Flavone could prevent the RPE from oxidation-induced injury both in vivo and in vitro.

Keywords: age-related macular degeneration; flavone; oxidation; retinal pigment epithelium.

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Figures

Figure 1
Figure 1. Representative ERG wave form at 4 weeks after NaIO3 injection
A: vehicle control group; B: NaIO3 group; C: flavone+NaIO3 group
Figure 2
Figure 2. Effect of flavone on proliferation of ARPE-19 cells
ARPE-19 cells were incubated with flavone for 72 hours. n=6 in each group; bP<0.01 vs vehicle control group
Figure 3
Figure 3. Effect of flavone on hypoxia-induced injury in ARPE-19 cells
ARPE-19 cells were incubated with flavone for 72 hours. Control group treated with vehicle (30% HP-β-CD solution) under normal condition (50mL/L CO2 and 950mL/L air) for 72 hours; model group treated with vehicle under hypoxic condition (10mL/L O2, 50mL/L CO2 and 940mL/L N2) for 72 hours. n=6 in each group; aP<0.05 and bP<0.01 vs model group
Figure 4
Figure 4. Effect of flavone on H2O2-induced injury in ARPE-19 cells
ARPE-19 cells were incubated with flavone and H2O2 for 24 hours. n =6 in each group; aP<0.05 and bP<0.01 vs model group
Figure 5
Figure 5. Effect of flavone on NaN3-induced injury in ARPE-19 cells
ARPE-19 cells were incubated with flavone and NaN3 for 72 hours. n =6 in each group; aP <0.05 and bP <0.01 vs model group
Figure 6
Figure 6. Effect of flavone on t-BHP-induced injury in ARPE-19 cells
ARPE-19 cells were incubated with flavone and t-BHP for 12 hours. n=6 in each group; aP<0.05 and bP<0.01 vs model group
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