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. 2011;4(1):19-25.
doi: 10.3980/j.issn.2222-3959.2011.01.04. Epub 2011 Feb 18.

Effect of CD4(+)CD25(+) regulatory T cells in the development of anterior chamber-associated immune deviation

Shu-Xing Ji  1 , Xiao-Lei Yin, Pei-Zeng Yang
Affiliations

Effect of CD4(+)CD25(+) regulatory T cells in the development of anterior chamber-associated immune deviation

Shu-Xing Ji et al. Int J Ophthalmol. 2011.

Abstract

Aim: To investigate whether CD4(+)CD25(+) regulatory T (Treg) cells play a role in the development of anterior chamber-associated immune deviation (ACAID).

Methods: The dynamic changes in the frequency of CD4(+)CD25(+) T cells, CD4(+)CD25(+) FoxP3(+) T cells and CD4(+)CD25(+) PD-1(+) T cells from spleens of mice with ACAID were analyzed by flow cytometry. Foxp3 mRNA expression in purified CD4(+)CD25(+) T cells was analyzed using real-time PCR. The suppressive effect of purified CD4(+)CD25(+) T cells on the proliferation of CD4(+)CD25(-) T cells was evaluated by [(3)H] thymidine incorporation. A blocking experiment was performed to further address the role of CD4(+)CD25(+) T cells in ACAID. The expression of IL-10 in purified CD4(+)CD25(+) T cells was evaluated by ELISA.

Results: Increased frequencies of CD4(+)CD25(+) T cells, CD4(+)CD25(+) FoxP3(+) T cells and CD4(+)CD25(+) PD-1(+) T cells were observed in ACAID. The CD4(+)CD25(+) T cells from mice with ACAID showed enhanced suppressive effect on the proliferation of CD4(+)CD25(-) T cells. Treatment of BALB/c mice with anti-CD25 antibody after injection of OVA into the anterior chamber significantly inhibited the induction of ACAID. Furthermore, purified CD4(+)CD25(+) T cells from ACAID mice secreted IL-10.

Conclusion: Our results demonstrate that Treg cells are induced in the mice undergoing ACAID. These Treg cells may play a role in the development of ACAID.

Keywords: ACAID; CD4+CD25+ regulatory T cells; Foxp3; IL-10; PD-1.

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Figures

Figure 1
Figure 1. Foxp3 mRNA expression
bP<0.01 vs three controls
Figure 2
Figure 2. [3H] thymidine incorporation
Figure 3
Figure 3. Effect of CD 25+ cell depletion
bP<0.01 vs ACAID, Isotype control and Positive control
Figure 4
Figure 4. IL-10 production “+” meaning in the presence of , “-” meaning in the absence of
See this image and copyright information in PMC

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