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. 2011;4(6):590-3.
doi: 10.3980/j.issn.2222-3959.2011.06.03. Epub 2011 Dec 18.

Investigation of immunogenicity of cryopreserved limbal stem cells

Yan Zhang  1 , Cui-Xia Li, Li Sun
Affiliations

Investigation of immunogenicity of cryopreserved limbal stem cells

Yan Zhang et al. Int J Ophthalmol. 2011.

Abstract

Aim: To investigate changes in immunogenicity of cryopreserved limbal stem cells.

Methods: Cryopreserved limbal stem cells, fresh primary limbal stem cells and blank controls were inoculated subcutaneously in C57BL-6 mice and the percentage of CD25 cells in limbal explants was determined by flow cytometry at day 21 post inoculation. Morphological studies were performed by light and electron microscopy of limbal explant sections.

Results: The number of regional and systemic lymphocytes derived from cryopreserved limbal stem cells was lower than that from fresh primary limbal stem cells.

Conclusion: Lymphocytes derived from cryopreserved limbal stem cells showed changes in immunogenicity, but the significance is unknown. The cryopreservation and thawing methods await further study.

Keywords: cryopreservation; explant; immunogenicity; rabbit limbal stem cells.

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Figures

Figure 1
Figure 1. Light microscopy of mice subcutaneous derived from
A: Blank control; B: Cryopreserved rabbit limbal explants; C: Fresh primary rabbit limbal explants
Figure 2
Figure 2. Electron microscopy of mice subcutaneous derived from
A:Blank control; B: Cryopreserved rabbit limbal explants; C: Fresh primary rabbit limbal explants
Figure 3
Figure 3. Analysis of mice blood cells from cryopreserved rabbit limbal explants, fresh primary rabbit limbal explants or blank controls cultured for three weeks by flow cytometry for the percentage of CD3+/CD25+ cells. Single cell suspensions were analyzed according to their size (FSC) and granularity (SSC) profiles
A: Cryopreserved rabbit limbal explants; B: Fresh primary rabbit limbal explants; C: Controls.
See this image and copyright information in PMC

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