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. 2012;5(1):7-12.
doi: 10.3980/j.issn.2222-3959.2012.01.02. Epub 2012 Feb 18.

Inhibited experimental corneal neovascularization by neutralizing anti-SDF-1α antibody

Gao-Qin Liu  1 , Pei-Rong Lu, Long-Biao Li, Xue-Guang Zhang
Affiliations

Inhibited experimental corneal neovascularization by neutralizing anti-SDF-1α antibody

Gao-Qin Liu et al. Int J Ophthalmol. 2012.

Abstract

Aim: To explore the effect of SDF-1α on the development of experimental corneal neovascularization (CRNV).

Methods: CRNV was induced by alkali injury in mice. The expression of SDF-1α and CXCR4 in burned corneas was examined by Flow Cytometry. Neutralizing anti-mouse SDF-1α antibody was locally administrated after alkali injury and the formation of CRNV 2 weeks after injury was assessed by Immunohistochemistry. The expression of VEGF and C-Kit in burned corneas was detected by RT-PCR.

Results: The number of CRNV peaks at 2 weeks after alkali injury. Compared to control group, SDF-1α neutralizing antibody treatment significantly decreased the number of CRNV. RT-PCR confirmed that SDF-1α neutralizing antibody treatment resulted in decreased intracorneal VEGF and C-Kit expression.

Conclusion: SDF-1α neutralizing antibody treated mice exhibited impaired experimental CRNV through down regulated VEGF and C-Kit expression.

Keywords: alkali injury; chemokine; corneal neovascularization.

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Figures

Figure 1
Figure 1. SDF-1α and CXCR4 protein expression in normal and alkali injured corneas.
Figure 2
Figure 2. Alkali injury-induced CRNV in anti-SDF-1α mAbs treated and vehicle treated BALB/c mice
A, B: Macroscopic appearance of eyes from vehicle and anti-SDF-1α mAbs treated BALB/c mice 2 weeks after alkali injury; C, D: Tissues were immunostained with anti-CD31 antibody, and representative results are shown here. Original magnifications×400; E: CRNV numbers per square millimeter in whole section; F: Percentage CRNV areas in hot spots were determined from the corneas obtained from vehicle and anti-SDF-1α mAbs treated BABL/c mice 2 weeks after injury. Each value represents mean±SEM (n=5-8 animals). aP<0.05 vs vehicle-treated mice.
Figure 3
Figure 3. RT-PCR analysis of gene expression in the injured corneas of vehicle and anti-SDF-1α mAbs treated BALB/c mice
A: Representative result from three independent experiments of RT-PCR; B-E: Ratios of indicated genes to GAPDH of alkali-injured vehicle (black bar) and anti-SDF-1α mAbs treated BALB/c mice (grey bar) determined by RT-PCR at the indicated time intervals after alkali injury. All values represent mean±SEM (n=5-8 animals). aP<0.05 vs vehicle-treated mice.
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