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Review

Impedance-Based Technologies

Kim E. Garbison  1 , Beverly A. Heinz  1 , Mary E. Lajiness, Jeffrey R. Weidner  2 , G. Sitta Sittampalam  3
Sarine Markossian  1 , Abigail Grossman  1 , Hannah Baskir  1 , Michelle Arkin  2 , Douglas Auld  3 , Christopher Austin  4 , Jonathan Baell  5 , Kyle Brimacombe  1 , Thomas D.Y. Chung  6 , Nathan P. Coussens  7 , Jayme L. Dahlin  8 , Viswanath Devanarayan  9 , Timothy L. Foley  10 , Marcie Glicksman  11 , Kirill Gorshkov  12 , Stefan Grotegut  13 , Matthew D. Hall  1 , Samuel Hoare  14 , James Inglese  1 , Philip W. Iversen  15 , Madhu Lal-Nag  16 , Zhuyin Li  12 , Jason R. Manro  13 , James McGee  17 , Allison Norvil  13 , Mackenzie Pearson  13 , Terry Riss  18 , Peter Saradjian  19 , G. Sitta Sittampalam  20 , Michael A. Tarselli  21 , O. Joseph Trask Jr.  22 , Jeffrey R. Weidner  23 , Mary Jo Wildey  24 , Kelli Wilson  1 , Menghang Xia  1 , Xin Xu  1
, editors.
In: Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004.
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Review

Impedance-Based Technologies

Kim E. Garbison et al.
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Excerpt

Activation of endogenous receptors (GPCR, tyrosine kinase and nuclear receptors) results in changes in the cytoskeleton and related morphological changes in cells. Changes in electrical impedance associated with these morphological changes in the presence of agonists and antagonists of these receptors can be used to evaluate hits from HTS assays. In this chapter, the basics of impedance assays and the use of two instruments available for these applications are discussed. Examples of GPCR activation and a sample protocol are also provided for the benefit of beginners and experienced investigators.

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References

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