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Review

Phospho-ERK Assays

Kim E. Garbison  1 , Beverly A. Heinz, Mary E. Lajiness
Jeffrey R. Weidner, G. Sitta Sittampalam
, editors.
Sarine Markossian  1 , Abigail Grossman  1 , Hannah Baskir  1 , Michelle Arkin  2 , Douglas Auld  3 , Christopher Austin  4 , Jonathan Baell  5 , Kyle Brimacombe  1 , Thomas D.Y. Chung  6 , Nathan P. Coussens  7 , Jayme L. Dahlin  8 , Viswanath Devanarayan  9 , Timothy L. Foley  10 , Marcie Glicksman  11 , Kirill Gorshkov  12 , Stefan Grotegut  13 , Matthew D. Hall  1 , Samuel Hoare  14 , James Inglese  1 , Philip W. Iversen  15 , Madhu Lal-Nag  16 , Zhuyin Li  12 , Jason R. Manro  13 , James McGee  17 , Allison Norvil  13 , Mackenzie Pearson  13 , Terry Riss  18 , Peter Saradjian  19 , G. Sitta Sittampalam  20 , Michael A. Tarselli  21 , O. Joseph Trask Jr.  22 , Jeffrey R. Weidner  23 , Mary Jo Wildey  24 , Kelli Wilson  1 , Menghang Xia  1 , Xin Xu  1
, editors.
In: Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004.
[updated ].
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Review

Phospho-ERK Assays

Kim E. Garbison et al.
Free Books & Documents

Excerpt

Extracellular signal-related kinase (ERK1/2 or p42/44) is a kinase in the mitogen-activated protein kinase (MAPK) family and phosphorylation of ERK (p-ERK) can be used as a common end point measurement for the activation of many classes of G protein coupled receptors (GPCR). This chapter addresses the use of PerkinElmer’s AlphaScreen® SureFireTMassay to measure ERK phosphorylation. This chapter is intended to assist in the development and optimization of p-ERK assays by providing sample protocols, factors to consider during assay optimization and data analysis details for the agonist and antagonist modes.

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References

Literature Cited

    1. Osmond RIW, Sheehan A, Borowicz R, Barnett E, Harvey G, Turner C, Brown A, Crouch MF, Dyer AR. GPCR Screening via ERK 1/2: A Novel Platform for Screening G protein-Coupled Receptors. J. Biomol. Screen. 2005;10(7):730–737. - PubMed

Additional References

    1. Luttrell DK, Luttrell LM. Signaling in time and space: G protein-coupled receptors and mitogen-activated protein kinases. Assay Drug Dev Tech. 2003;1:327–338. - PubMed
    1. Luttrell LM. G protein-coupled receptor signaling in neuroendocrine systems. ‘Location, location, location’: activation and targeting of MAP kinases by G protein-coupled receptors. J. Molec. Endocrinology. 2003;30:117–126. - PubMed
    1. Schulte G, Fredholm BB. Signalling from adenosine receptors to mitogen-activated protein kinases. Cellular Signalling. 2003;15:813–827. - PubMed

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