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. 2012 May 3:11:11.
doi: 10.1186/1476-0711-11-11.

Isolation of antibacterial compounds from Quercus dilatata L. through bioassay guided fractionation

Maryam Jamil  1 , Ihsan ul Haq, Bushra Mirza, Mazhar Qayyum
Affiliations

Isolation of antibacterial compounds from Quercus dilatata L. through bioassay guided fractionation

Maryam Jamil et al. Ann Clin Microbiol Antimicrob. .

Abstract

Background: Four medicinal plants (Chrozophora hierosolymitana Spreng, Chrysanthemum leucanthemum L., Ephedra gerardiana Wall. ex Stapf, and Quercus dilatata L.) used by indigenous healers to treat various infectious diseases were selected for the present study. The major objective of the present study was isolation and characterization of antimicrobial components from the crude plant extracts using bioassay guided fractionation.

Methods: Seven methanolic extracts of the four plants were screened to identify any antimicrobial agents present in them. The active crude plant extract was fractionated first by solvent partitioning and then by HPLC. Characterization of the active fractions was done by using spectrophotometer.

Results: All the seven methanolic extracts showed low antifungal activity, however, when these extracts were tested for antibacterial activity, significant activity was exhibited by two extracts. The extract of aerial parts of Q. dilatata was most active and therefore, was selected for further analysis. Initially fractionation was done by solvent-solvent partitioning and out of six partitioned fractions, ethanol fraction was selected on the basis of results of antibacterial activity and phytochemical analysis. Further, fractionation was carried out by RP-HPLC and purified active subfractions were characterized by comparing their absorption spectra with that of the known natural products isolated from the plants of Quercus genus.

Discussion and conclusion: The results suggest that this is the first report of the isolated antibacterial compounds from this genus.

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Figures

Figure 1
Figure 1
Scheme of fractions preparation.
Figure 2
Figure 2
Preparative HPLC Chromatogram obtained for AM3b fraction showing single peak at 2.992 min.
Figure 3
Figure 3
Preparative HPLC Chromatogram obtained for AM3c fraction showing single peak at 6.957 min.
Figure 4
Figure 4
Comparison of absorption spectrum of AM3b with that of standard compounds previously isolated from Quercus genus showing that AM3b does not match with any of these compounds.
Figure 5
Figure 5
Comparison of absorption spectrum of AM3c with that of standard compounds previously isolated from Quercus genus showing that AM3c does not match with any of these compounds.
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