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Comparative Study
. 1990 Dec 7;250(4986):1426-9.
doi: 10.1126/science.2255914.

Disruption of the human SCL locus by "illegitimate" V-(D)-J recombinase activity

P D Aplan  1 , D P Lombardi, A M Ginsberg, J Cossman, V L Bertness, I R Kirsch
Affiliations
Comparative Study

Disruption of the human SCL locus by "illegitimate" V-(D)-J recombinase activity

P D Aplan et al. Science. .

Abstract

A fusion complementary DNA in the T cell line HSB-2 elucidates a provocative mechanism for the disruption of the putative hematopoietic transcription factor SCL. The fusion cDNA results from an interstitial deletion between a previously unknown locus, SIL (SCL interrupting locus), and the 5' untranslated region of SCL. Similar to 1;14 translocations, this deletion disrupts the SCL 5' regulatory region. This event is probably mediated by V-(D)-J recombinase activity, although neither locus is an immunoglobulin or a T cell receptor. Two other T cell lines, CEM and RPMI 8402, have essentially identical deletions. Thus, in lymphocytes, growth-affecting genes other than immune receptors risk rearrangements.

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