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. 2013 Feb;15(1):79-86.
doi: 10.1007/s11307-012-0561-3.

Imaging of protein synthesis: in vitro and in vivo evaluation of (44)Sc-DOTA-puromycin

Sebastian Eigner  1 , Denis R Beckford Vera, Marco Fellner, Natalia S Loktionova, Markus Piel, Ondrej Lebeda, Frank Rösch, Tobias L Roß, Katerina Eigner Henke
Affiliations

Imaging of protein synthesis: in vitro and in vivo evaluation of (44)Sc-DOTA-puromycin

Sebastian Eigner et al. Mol Imaging Biol. 2013 Feb.

Abstract

Purpose: The purpose of this study was to investigate whether (44)Sc-labeled puromycin can be utilized for imaging of protein synthesis in vivo.

Methods: For micro-positron emission tomographic (μPET) studies, 20-25 MBq of [(44)Sc]-DOTA-puromycin was administered to tumor-bearing rats, and animals were scanned for 1 h dynamically. Results were further validated by dissecting organs and tissues of the animals after the measurement and in vitro blocking experiments using puromycin or cycloheximide to block protein synthesis.

Results: μPET images of tumor-bearing rats showed significant tumor uptake of [(44)Sc]-DOTA-puromycin and a clear-cut tumor visualization. In both blocking experiments, cellular uptake of [(44)Sc]-DOTA-puromycin ([(44)Sc]-DOTA-Pur) could be suppressed by blocking protein synthesis.

Conclusions: We report for the first time successful μPET imaging with (44)Sc obtained from a (44)Ti/(44)Sc generator, as well as noninvasive μPET imaging of ribosomal activity, respectively protein synthesis, with a puromycin-based radiopharmaceutical and the direct correlation between cellular uptake of [(44)Sc]-DOTA-Pur and protein synthesis.

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References

    1. Radiother Oncol. 2010 Sep;96(3):280-7 - PubMed
    1. Eur J Nucl Med Mol Imaging. 2004 Aug;31(8):1097-104 - PubMed
    1. J Nucl Med. 1994 Feb;35(2):317-25 - PubMed
    1. FEBS Lett. 1999 Nov 26;462(1-2):43-6 - PubMed
    1. J Nucl Med. 2004 Feb;45(2):347-55 - PubMed

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