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Editorial
. 2012 May;5(3):289-92.
doi: 10.1242/dmm.009878.

Towards an encyclopaedia of mammalian gene function: the International Mouse Phenotyping Consortium

Steve D M Brown, Mark W Moore
Editorial

Towards an encyclopaedia of mammalian gene function: the International Mouse Phenotyping Consortium

Steve D M Brown et al. Dis Model Mech. 2012 May.
No abstract available

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Figures

Fig. 1.
Fig. 1.
Organisation of the IMPC. A core of mouse production and phenotyping centres is allied to the data centre supporting the IMPC. The core mouse production and phenotyping centres also network with a variety of other biomedical research centres that will undertake more specialised phenotyping on selected lines, as well as provide additional expertise and input into the IMPC phenotyping programme. COPD, chronic obstructive pulmonary disease; CVD, cardiovascular disease; Dev biol, developmental biology; KO, knockout; Neuro, neurological; QC, quality control.
Fig. 2.
Fig. 2.
Design of the knockout first, conditional ready allele employed by the IMPC. The tm1a allele is generated in ES cells (which itself provides a knockout through splicing into the lacZ reporter). Mice generated with the tm1a allele are crossed to an appropriate Cre driver line to both eliminate a key exon within the gene and remove the selection cassette, generating the tm1b null allele. The tm1b allele will be phenotyped by the IMPC. Alternatively, Flp action on the tm1a allele can produce the tm1c ‘conditional ready’ allele. Grey boxes represent exons, and grey lines show introns. Figure reproduced from Skarnes et al. (Skarnes et al., 2011) with permission.
Fig. 3.
Fig. 3.
The IMPC adult phenotyping pipeline. The consensus IMPC pipeline for phenotyping in the adult is shown, highlighting mandatory tests, non-mandatory tests (which a number of centres will employ, but not all) and tests under development. In-life phenotyping is carried out from 9 to 15 weeks, after which a variety of terminal tests are employed. ECG, electrocardiogram; Echo, echocardiogram; F, female; M, male; PPI, prepulse inhibition. Schematic reproduced from www.mousephenotype.org, with permission.
See this image and copyright information in PMC

References

    1. Brown S. D. M., Hanckcok J. M., Gates H. (2006). Understanding mammalian genetic systems: The challenge of phenotyping in the mouse. PLoS Genet. 2, e118. - PMC - PubMed
    1. Eppig J. T., Blake J. A., Bult C. J., Kadin J. A., Richardson J. E. (2012). The Mouse Genome Database (MGD): comprehensive resource for genetics and genomics of the laboratory mouse. Nucleic Acids Res. 40, D881–D886 - PMC - PubMed
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    1. Schofield P. N., Vogel P., Gkoutos G. V., Sundberg J. P. (2012). Exploring the elephant: histopathology in the high-throughput phenotyping of mutant mice. Dis. Model. Mech. 5, 19–25 - PMC - PubMed
    1. Skarnes W. C., Rosen B., West A. P., Koutsourakis M., Bushell W., Iyer V., Mujica A. O., Thomas M., Harrow J., Cox T., et al. (2011). A conditional knockout resource for the genome-wide study of mouse gene function. Nature 474, 337–342 - PMC - PubMed

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