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. 2012:2012:987508.
doi: 10.1100/2012/987508. Epub 2012 Apr 1.

Beneficial effects of montelukast against methotrexate-induced liver toxicity: a biochemical and histological study

Evren Kose  1 , Hilal Irmak Sapmaz, Ediz Sarihan, Nigar Vardi, Yusuf Turkoz, Nihat Ekinci
Affiliations

Beneficial effects of montelukast against methotrexate-induced liver toxicity: a biochemical and histological study

Evren Kose et al. ScientificWorldJournal. 2012.

Abstract

The effects of montelukast against methotrexate-induced liver damage were investigated. 35 Wistar albino female rats were divided into 5 groups as follows: group I: control; group II: montelukast (ML); group III: methotrexate (Mtx); group IV: montelukast treatment after methotrexate application (Mtx + ML); group V: montelukast treatment before methotrexate application (ML + Mtx). At the end of the experiment, the liver tissues of rats were removed. Malondialdehyde (MDA), myeloperoxidase (MPO), and reduced glutathione levels were determined from liver tissues. In addition, the liver tissues were examined histologically. MDA and MPO levels of Mtx group were significantly increased when compared to control group. In Mtx + ML group, these parameters were decreased as compared to Mtx group. Mtx injection exhibited major histological alterations such as eosinophilic staining and swelling of hepatocytes. The glycogen storage in hepatocytes was observed as decreased by periodic acid schiff staining in Mtx group as compared to controls. ML treatment did not completely ameliorate the lesions and milder degenerative alterations as loss of the glycogen content was still present. It was showed that montelukast treatment after methotrexate application could reduce methotrexate-induced experimental liver damage.

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Figures

Figure 1
Figure 1
(a) Normal histological appearance of liver in the control group. VC: Vena centralis. (b) The PAS-positive reaction shows a magenta staining where glycogen is present within hepatocytes. X66.
Figure 2
Figure 2
(a) Hepatocytes with eosinophilic cytoplasm (arrows) are observed in Mtx group. (b) Cellular swelling in hepatocytes (arrows) is noticed in Mtx group. (c) Marked reduction in glycogen content in Mtx group. X66.
Figure 3
Figure 3
(a) Notice decreased glycogen storage in hepatocytes of Mtx + ML group. (b) View of glycogen storage in hepatocytes in ML + Mtx group. (c) The appearance of the swollen hepatocyte in Mtx + ML group (arrows). (d) Eosinophilic-stained hepatocytes are still observed (arrows) in ML + Mtx group. X66.
See this image and copyright information in PMC

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