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. 2011 Aug 15;4(3):250-5.
Epub 2011 Aug 25.

In vitro biocompatibility testing of some synthetic polymers used for the achievement of nervous conduits

R Mihai  1 , I P Florescu, V Coroiu, A Oancea, M Lungu
Affiliations

In vitro biocompatibility testing of some synthetic polymers used for the achievement of nervous conduits

R Mihai et al. J Med Life. .

Abstract

Biocompatible synthetic polymers are largely used in the bio-medical domain, tissue engineering and in controlled release of medicines. Polymers can be used in the achievement of cardiac and vascular devices, mammary implants, eye lenses, surgical threads, nervous conduits, adhesives, blood substitutes, etc. Our study was axed on the development of cytotoxicity tests for 3 synthetic polymers, namely polyvinyl alcohol, polyethylene glycol and polyvinyl chloride. These tests targeted to determine the viability and morphology of cells (fibroblasts) that were in indirect contact with the studied polymers. Cell viability achieved for all the studied synthetic polymers allowed their frame in biocompatible material category. Cell morphology did not significantly change, thus accomplishing a new biocompatibility criterion. The degree of biocompatibility of the studied polymers varied. Polyvinyl alcohol presented the highest grade of biocompatibility and polyvinyl chloride placed itself at the lowest limit of biocompatibility. The results achieved allowed the selection of those polymers that (by enhancing their degrees of biocompatibility due to the association with various biopolymers) will be used in the development of new biocompatible materials, useful in nervous conduits manufacture.

Keywords: MTT test; Polyvinyl alcohol; dermal fibroblasts; polyethylenglycol; polyvinyl chloride.

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Figures

Figure 1
Figure 1
Fibroblast viability in presence of PVC extract after 24 and 48 hours of culture
Figure 2
Figure 2
Light microscopy images of cell cultures after 24 hours. A –control sample; B – PVC sample
Figure 3
Figure 3
Cell proliferation test (MTT) at 24 and 48 hours in the presence of APV extract
Figure 4
Figure 4
Light microscopy images of cell cultures after a 48 hours period of culture with the APV extract (B) and without it (control sample – A).
Figure 5
Figure 5
Cell proliferation test (MTT) at 24 and 48 hours
Figure 6
Figure 6
Light microscopy images of cell cultures after 48 hours for PEG extract (B) and for the control sample (A).
See this image and copyright information in PMC

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