Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012:2012:257109.
doi: 10.1155/2012/257109. Epub 2012 Apr 5.

Online Coupling of Lab-on-Valve Format to Amperometry Based on Polyvinylpyrrolidone-Doped Carbon Paste Electrode and Its Application to the Analysis of Morin

Yang Wang  1 , Guojun Yao, Jie Tang, Chun Yang, Qin Xu, Xiaoya Hu
Affiliations

Online Coupling of Lab-on-Valve Format to Amperometry Based on Polyvinylpyrrolidone-Doped Carbon Paste Electrode and Its Application to the Analysis of Morin

Yang Wang et al. J Anal Methods Chem. 2012.

Abstract

The potential capabilities and analytical performance of lab-on-valve (LOV) manifold as a front end to amperometry have been explored for the on-line determination of morin. Meanwhile, the electrochemical behaviors of morin were investigated based on polyvinylpyrrolidone- (PVP-) doped carbon paste electrode (CPE), which found that PVP can significantly improve its oxidation peak current. The excellent amperometric current response was achieved when the potential difference (ΔE) of 0.6 V was implemented in pH 6.5 phosphate buffer solution (PBS) that served as the supporting electrolyte. A well-defined oxidation peak has been obtained in studies using PVP as a modifier of CPE based on the oxidation of morin. The present work introduces the LOV technique as a useful tool for amperometric measurement, documents advantages of using programmable flow, and outlines means for miniaturization of assays on the basis of PVP modified CPE. The proposed method was applied successfully to the determination of morin in real samples, and the spiked recoveries were satisfactory.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The structures of PVP (a) and morin (b).
Figure 2
Figure 2
Schematic diagram of the SI-LOV manifold in conjunction with amperometry for the determination of morin.
Figure 3
Figure 3
Cyclic voltammograms obtained at bare CPE (b) and PVP/CPE (a) surfaces using 1.0 × 10−5 mol L−1 morin solution in 0.05 mol L−1 PBS (pH 6.5).
Figure 4
Figure 4
Cyclic voltammograms obtained at PVP/CPE in 0.05 mol L−1 PBS with various scan rates. The scan rates were 25, 50, 100, 150, 200, 250, 300 mV/s from a to g. Inset: plot of the peak current as a function of the scan rate.
Figure 5
Figure 5
The amperometric current response with increasing concentrations of morin. ΔE of 0.6 V was applied. Sample volume was 200 μL with the flow rate of 30 μL s−1.
See this image and copyright information in PMC

References

    1. Jones SA, Martin GP, Brown MB. Determination of polyvinylpyrrolidone using high-performance liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis. 2004;35(3):621–624. - PubMed
    1. Yang X, Wang F, Hu S. The electrochemical oxidation of troxerutin and its sensitive determination in pharmaceutical dosage forms at PVP modified carbon paste electrode. Colloids and Surfaces B. 2006;52(1):8–13. - PubMed
    1. Guo H, Li Y, Xiao P, He N. Determination of trace amount of bismuth(III) by adsorptive anodic stripping voltammetry at carbon paste electrode. Analytica Chimica Acta. 2005;534(1):143–147.
    1. Ejhieh AN, Masoudipour N. Application of a new potentiometric method for determination of phosphate based on a surfactant-modified zeolite carbon-paste electrode (SMZ-CPE) Analytica Chimica Acta. 2010;658(1):68–74. - PubMed
    1. Shams E, Torabi R. Determination of nanomolar concentrations of cadmium by anodic-stripping voltammetry at a carbon paste electrode modified with zirconium phosphated amorphous silica. Sensors and Actuators B. 2006;117(1):86–92.