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. 2012 Apr;6(2):210-7.
doi: 10.5009/gnl.2012.6.2.210. Epub 2012 Apr 17.

Comparison of Indomethacin, Diclofenac and Aspirin-Induced Gastric Damage according to Age in Rats

Pyoung Ju Seo  1 , Nayoung Kim, Joo-Hyon Kim, Byoung Hwan Lee, Ryoung Hee Nam, Hye Seung Lee, Ji Hyun Park, Mi Kyoung Lee, Hyun Chang, Hyun Chae Jung, In Sung Song
Affiliations

Comparison of Indomethacin, Diclofenac and Aspirin-Induced Gastric Damage according to Age in Rats

Pyoung Ju Seo et al. Gut Liver. 2012 Apr.

Abstract

Background/aims: Aging gastric mucosa is known to have decreased mucosal defenses and increased susceptibility to injury by nonsteroidal anti-inflammatory drugs. Depending on the type of nonsteroidal anti-inflammatory drug (NSAID), the underlying mechanisms and the extent of damage to the stomach or intestine may differ. This study was performed to evaluate the acute gastric damage caused by different doses of indomethacin, diclofenac and aspirin in rats of various ages.

Methods: For the acute models, indomethacin (10, 20 or 40 mg/kg), diclofenac (40 or 80 mg/kg) or aspirin (100 mg/kg) was given to 7- and 25-week-old and 1-year-old Sprague-Dawley rats by intragastric gavage. The gross ulcer index, damage area as assessed by imaging, histological index, myeloperoxidase (MPO) activity, and cytosolic phospholipase A(2) (cPLA(2)) levels were measured after 24 hours.

Results: The gross ulcer index and damage area increased with age in the presence of three NSAIDs (p<0.05). The increases in MPO levels induced by diclofenac and aspirin were significantly higher in 1-year-old than 7-week-old rats (p<0.05). cPLA(2) expression induced by indomethacin (10 and 40 mg/kg) was greater in the 1-year-old rats, compared with 7-week-old rats (p<0.05).

Conclusions: NSAID-induced acute gastric damage increased in a dose- and age-dependent manner.

Keywords: Aging; Aspirin; Gastric damage; Nonsteroidal anti-inflammatory drugs.

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Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1
Fig. 1
Gross findings of the gastric damage caused by indomethacin (10 mg/kg), diclofenac (40 mg/kg), and aspirin (100 mg/kg) in rats. Indo, indomethacin; DF, diclofenac; Asp, aspirin.
Fig. 2
Fig. 2
(A) Gross ulcer index and (B) damaged area in the rat stomachs (7- and 25-week-old and 1-year-old rats) assessed by the image program. Mean±standard error with seven rats per group. Indo10, indomethacin 10 mg/kg; Indo20, indomethacin 20 mg/kg; Indo40, indomethacin 40 mg/kg; DF40, diclofenac 40 mg/kg; DF80, diclofenac 80 mg/kg; Asp 100, aspirin 100 mg/kg. *p<0.05 compared with the 7-week-old rats in the same nonsteroidal anti-inflammatory drug-treatment group.
Fig. 3
Fig. 3
Histological index in the rat stomachs (7- and 25-week-old and 1-year-old rats). Mean±standard error with seven rats per group. Indo10, indomethacin 10 mg/kg; Indo20, indomethacin 20 mg/kg; Indo40, indomethacin 40 mg/kg; DF40, diclofenac 40 mg/kg; DF80, diclofenac 80 mg/kg; Asp 100, aspirin 100 mg/kg.
Fig. 4
Fig. 4
Mucosal concentrations of (A) myeloperoxidase (MPO) and (B) cPLA2 expression in the rat stomachs (7- and 25-week-old and 1-year-old rats). Mean±standard error with seven rats per group. Indo10, indomethacin 10 mg/kg; Indo20, indomethacin 20 mg/kg; Indo40, indomethacin 40 mg/kg; DF40, diclofenac 40 mg/kg; DF80, diclofenac 80 mg/kg; Asp100, aspirin 100 mg/kg. *p<0.05 compared with the 7-week-old rats in the same nonsteroidal anti-inflammatory drug-treatment group.
See this image and copyright information in PMC

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