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Review
. 2012 Oct;113(10):3061-8.
doi: 10.1002/jcb.24183.

Assessing iPSC reprogramming methods for their suitability in translational medicine

Mahendra S Rao  1 , Nasir Malik
Affiliations
Review

Assessing iPSC reprogramming methods for their suitability in translational medicine

Mahendra S Rao et al. J Cell Biochem. 2012 Oct.

Abstract

The discovery of the ability to induce somatic cells to a pluripotent state through the overexpression of specific transcription factors has the potential to transform the ways in which pharmaceutical agents and cellular transplantation therapies are developed. Proper utilization of the technology to generate induced pluripotent stem cells (iPSCs) requires that researchers select the appropriate reprogramming method for generating iPSCs so that the resulting iPSCs can be transitioned towards clinical applications effectively. This article reviews all of the currently available reprogramming techniques with a focus on critiquing them on the basis of their utility in translational medicine.

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Figures

Fig. 1
Fig. 1
The process of generating translational grade iPSCs. The tissue of choice is selected to reprogram, by excisable or integration free methods to generate minimal- or zero-footprints iPSCs which are then ready for high throughput drug screens and/or sources for the derivation of cells for cellular transplantation therpies.
See this image and copyright information in PMC

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