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. 2010 May;2(5):241-5.
doi: 10.4297/najms.2010.2241.

Double staining immunohistochemistry

Xiao Chen  1 , Dan-Bi Cho, Ping-Chang Yang
Affiliations

Double staining immunohistochemistry

Xiao Chen et al. N Am J Med Sci. 2010 May.

Abstract

This article summarized immunohistochemistry methods generally used in research laboratories and clinic including direct immune staining, indirect immune staining, enzyme method, fluorescence method, APC method and PAP method.

Keywords: Immunohistochemistry.

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Figures

Fig. 1
Fig. 1
Making OCT blocks. A-C, making a mold with tinfoil. D, fill the mold with OCT. E, insert tissue into the bottom of mold. F, an OCT block after frozen in liquid nitrogen.
Fig. 2
Fig. 2
Comparing IHC images with or without blocking. Intestinal segments were processed for IHC with or without blocking. Two representative images show the effect of blocking. The non-blocked image show the results of staining in which not only very dirty, but also no positive cells were stained.
Fig. 3
Fig. 3
Indirect staining results. Intestinal segments were processed for IHC to stain neural tissue with anti-PGP9.5 antibodies labeled with FITC or Cy5.
Fig. 4
Fig. 4
PAP staining shows T cell and B cell in intestinal tissue. Colonic biopsy was processed for localization of B cells and T cells in the intestine by PAP method as described above.
Fig. 5
Fig. 5
Double staining colocalizes ChAT and 5-HTR2 on vagus neurons. Cultured vagus neurons were stained with anti-chloline acetyltransferase (ChAT) and anti-5-HTR2 antibodies with the procedures described above.
See this image and copyright information in PMC

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