The TL region gene 37 encodes a Qa-1 antigen
- PMID: 2258708
- PMCID: PMC2188773
- DOI: 10.1084/jem.172.6.1795
The TL region gene 37 encodes a Qa-1 antigen
Abstract
Of all the biochemically defined mouse MHC class I molecules, the Qa-1 antigens are the only ones for which a gene has not been identified. Recent evidence has suggested that Qa-1 antigens are functional class I molecules and can function as restriction elements for gamma/delta T cells. We have examined the relationship between Qa-1 and the product of gene 37, a presumed novel class I antigen encoded within the TL region. Immunoprecipitation and polyacrylamide gel electrophoresis analysis of the molecules reactive with anti-Qa-1 and anti-37 sera show that the Qa-1 molecule of Qa-1b (Qa-1.2) mouse strains is identical to the product of gene 37 on the basis of molecular weight, pI, and strain distribution. Immunodepletion, biosynthetic labeling, and tunicamycin treatment confirm that the protein encoded by gene 37 in Qa-1b mice is Qa-1.2. In contrast, the anti-37 serum was unable to recognize the Qa-1 molecule in Qa-1a strains. Given the fact that the only allele to gene 37 thus far identified in a Qa-1a strain (A/J) has a termination codon in the alpha 3 domain, our data lead us to conclude that the Qa-1 molecule expressed in Qa-1a mice is not a true allelic product of the gene 37 encoded antigen of Qa-1b mouse strains.
Similar articles
-
Heat shock proteins can regulate expression of the Tla region-encoded class Ib molecule Qa-1.Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10475-9. doi: 10.1073/pnas.88.23.10475. Proc Natl Acad Sci U S A. 1991. PMID: 1961712 Free PMC article.
-
Qa-1-associated antigens. IV. Evidence for additional Qa-1 polymorphism defined biochemically and by cytotoxic T lymphocyte recognition.Transplantation. 1982 Jul;34(1):54-9. Transplantation. 1982. PMID: 6181592
-
The Qa-1 alloantigens. II. Evidence for the expression of two Qa-1 molecules by the Qa-1d genotype and for cross-reactivity between Qa-1 and H-2K.J Immunol. 1983 Mar;130(3):1293-9. J Immunol. 1983. PMID: 6822734
-
Structure and function of H-2 T (Tla) region class I MHC molecules.Crit Rev Immunol. 1994;14(1):1-27. Crit Rev Immunol. 1994. PMID: 7741975 Review.
-
Qa/Tla antigens.Immunol Ser. 1989;43:379-98. Immunol Ser. 1989. PMID: 2490504 Review. No abstract available.
Cited by
-
Regulation of self-tolerance by Qa-1-restricted CD8(+) regulatory T cells.Semin Immunol. 2011 Dec;23(6):446-52. doi: 10.1016/j.smim.2011.06.001. Semin Immunol. 2011. PMID: 22136694 Free PMC article. Review.
-
Amino acid substitutions in the floor of the putative antigen-binding site of H-2T22 affect recognition by a gamma delta T-cell receptor.Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11396-400. doi: 10.1073/pnas.90.23.11396. Proc Natl Acad Sci U S A. 1993. PMID: 8248260 Free PMC article.
-
Transporters associated with antigen processing (TAP)-independent presentation of soluble insulin to alpha/beta T cells by the class Ib gene product, Qa-1(b).J Exp Med. 1998 Sep 7;188(5):961-71. doi: 10.1084/jem.188.5.961. J Exp Med. 1998. PMID: 9730897 Free PMC article.
-
Fine map of a region homologous to the rat grc complex in the TL region of the mouse.Immunogenetics. 1993;37(5):386-9. doi: 10.1007/BF00216804. Immunogenetics. 1993. PMID: 8428772 No abstract available.
-
The class I-b molecule Qa-1 forms heterodimers with H-2Ld and a novel 50-kD glycoprotein encoded centromeric to I-E beta.J Exp Med. 1995 Feb 1;181(2):657-68. doi: 10.1084/jem.181.2.657. J Exp Med. 1995. PMID: 7836920 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
Miscellaneous