Radiofrequency ablation of the liver: effect of variation of portal venous blood flow on lesion size in an in-vitro perfused bovine liver

Abstract

Rationale and objectives: An in vitro perfused bovine liver model was used to evaluate the relationship between the sizes of radiofrequency ablation lesions and variation in portal venous blood flow.

Materials and methods: Fourteen bovine livers were perfused with autologous heparinized blood at 37°C and 40% to 50% oxygenation via the portal vein. Flow rates were adjusted from 10 to 50 mL/min/100 g tissue. A 480-kHz generator and a 3.0-cm monopolar internally cooled electrode were used to create 57 ablations. The long-axis diameter, short-axis diameter (SAD), and volume of each ablation zone were measured and calculated from the dissected livers. Correlations between SAD, long-axis diameter, and volume versus blood flow were assessed using linear regression analysis.

Results: SAD and lesion volume demonstrated inverse linear correlations with blood flow (for SAD, y = -0.044x + 3.925, r = 0.836, P < .001; for volume, y = -0.556x + 31.574, r = 0.842, P < .001). A 10 mL/min/100 g change in flow rate produced an average 4.4 ± 0.4 mm change in SAD and an average 5.6 ± 0.5 cm(3) change in volume. Long-axis diameter was not correlated with blood flow (y = -0.7694x + 4.1899, r = 0.2173, P = .111).

Conclusions: The SAD and volume of radiofrequency ablation lesions have statistically significant inverse linear correlations with portal venous blood flow, with an average 4.4-mm change in SAD and an average 5.6-cm(3) change in volume for each 10 mL/min/100 g change in flow rate.