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. 2012:18:1175-81.
Epub 2012 May 4.

Hyaluronic acid increases MMP-2 and MMP-9 expressions in cultured trabecular meshwork cells from patients with primary open-angle glaucoma

Mao-Sheng Guo  1 , Yu-Yu Wu, Zong-Bao Liang
Affiliations

Hyaluronic acid increases MMP-2 and MMP-9 expressions in cultured trabecular meshwork cells from patients with primary open-angle glaucoma

Mao-Sheng Guo et al. Mol Vis. 2012.

Abstract

Purpose: A depletion of hyaluronic acid (HA) in patients' eyes may be associated with primary open-angle glaucoma (POAG), but the exact mechanism remains unclear. We investigated the effect of HA on the expression of matrix metalloproteinases (MMP-2 and MMP-9) in cultured trabecular meshwork cells.

Methods: Trabecular meshwork cells were cultured from trabecular tissues obtained from the POAG patients aged 23 to 45. The mRNA expression of MMP-2 and MMP-9 was determined by reverse transcription-polymerase chain reaction, and the protein expression of MMP-2 and MMP-9 by gelatin zymography analysis and qualified by the gel electrophoresis image analysis in different HA concentrations.

Results: The expression of MMP-2 and MMP-9 by the two methods significantly increased with HA concentration in a dose-response manner. Mean values of the MMP-2 expression by the gelatin zymography analysis were 176, 264, 353, and 448 mg/ml, and mean values of the MMP-9 expression were 547, 659, 895, and 1,147 mg/ml, for HA concentration level of 0, 1, 3, and 6 mg/ml, respectively.

Conclusions: In POAG trabecular meshwork cells, the level of HA concentration increases the activities of MMP-2 and MMP-9. The lack of HA in aqueous humor can result in a reduction in activities of MMPs and therefore may be involved in the pathogenesis of POAG.

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Figures

Figure 1
Figure 1
FN staining, LM staining, NSE staining, and TEM image of trabecular meshwork cells. A: Fibronectin (FN) staining (400×). B: Laminin (LM) staining (400×). C: Neuron-specific enolase (NSE) staining (5,000×). D: Transmission electron microscopy (TEM) image.
Figure 2
Figure 2
Gel electrophoresis of MMP-2 mRNA and GAPDH mRNA PCR in cultured trabecular meshwork cells. MMP=Matrix metalloproteinases.
Figure 3
Figure 3
Gel electrophoresis of MMP-9 mRNA and ACTB mRNA PCR in cultured trabecular meshwork cells. MMP=Matrix metalloproteinases.
Figure 4
Figure 4
Spectral analysis of MMP-2 protein in trabecular meshwork supernatant. MMP=Matrix metalloproteinases.
Figure 5
Figure 5
Spectral analysis of MMP-9 protein in trabecular meshwork supernatant. MMP=Matrix metalloproteinases.
Figure 6
Figure 6
Gelatin zymography analysis showing a dose–response relationship of HA on the protein expression of MMP-9. MMP=Matrix metalloproteinases; HA=hyaluronic acid.
See this image and copyright information in PMC

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