[Establishment and characterization of a human lung adenocarcinoma cell line, Ch-Huang-1]

Abstract

Background and objective: Lung cancer is a major worldwide health problem. The aim of this study is to establish a novel Chinese human lung adenocarcinoma cell line and examine its biological characteristics.

Methods: Lung adenocarcinoma specimens were freshly resected during surgery. The tissues were incubated in vitro and the cell line was named Ch-Huang-1. The biological characteristics of the cells were investigated by light microscopy, chromosome analysis, and transplantation experiment.

Results: Light microscopy revealed that cells from the primary tumor, Ch-Huang-1 cell line, and transplanted tumor possessed the characteristics of a malignant glandular epithelial tumor. The cell growth curve, doubling time, and mitotic index were also observed in vitro. Nuclear chromosome analysis revealed that the tumor was a subtriploid with a mode of 35-44 per cell. Tumor nodes were observed under the skin of nude mice by heterogenic transplantation.

Conclusion: The characteristics of the established cell line suggest that it is a newly established human adenocarcinoma cell line.

1

倒置显微镜光镜观察：细胞多呈多角形，少数为梭形或圆形（×200）。A：原代培养细胞；B：传代细胞。 Most cells were polygonal and a few were spindle or round (×200). A: primary cultured cells; B: passage cells.

2

细胞生长曲线图 Growth curve of cells line Ch-Huang-1

3

细胞分裂指数分析 Analysis of cells division index

4

细胞组织起源鉴定（DAB，×200） Identification of the cellular tissue origin (DAB, ×200)

5

染色体核型分析 Chromosomal instability in Ch-Huang-1

6

移植瘤癌细胞生长旺盛（HE，×40）。A：原代培养用组织块；B：移植瘤细胞。 Vigorous growth of transplanted tumor cells (HE, ×40). A: primary tissue culture cells; B: transplantation tumor cells.