Histological evaluation of bone healing using organic bovine bone in combination with platelet-rich plasma (an experimental study on rabbits)

Abstract

Objectives: This study was carried out to histologically assess the effect of bone grafting materials extracted from bovine origin on the bone healing process either alone or when mixed with autologous platelet-rich plasma which could be used in many procedures of oral and maxillofacial bone and implant reconstructive surgery.

Materials and methods: Sixteen rabbits were used; three intrabony defects in the femur bone of each rabbit were created, one left unfilled for normal healing process and served as control, the second filled with xenogenic graft (Gen-Ox-lyophilized bovine bone organic matrix), and the third filled with (Gen-Ox-lyophilized bovine bone organic matrix) mixed with autologous platelet-rich plasma. Histological examination of the sections was performed after staining with H&E and Van Geison stains. The histomorphometric analysis including counting of bone cells (osteoblasts, osteocytes, and osteoclasts) with performance of osteon diameter and lamellar thickness at the end of the fourth week postoperatively was obtained.

Results: It has been shown that with the use of autologous platelet-rich plasma in combination with the xenogenic bone graft prepared from bovine origin, new bone formation and neovascularization were enhanced significantly when compared with xenogenic graft alone.

Conclusion: The addition of PRP to xenogenic bone substitute in small bone defects of the rabbit femur showed a histomorphometric increase in bone formation (at the fourth week of healing).

Clinical relevance: Platelet concentrate might be used to accelerate the osseointegration of enosseous dental implants.