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. 1990:7 Suppl 1:S162-7.
doi: 10.1080/02652039009373873.

Effect of vitamin A deficiency on the in vitro transfer of mannose and N-acetylglucosamine in rat liver nuclei

S Galland  1 , Y Fayet, A Degiuli, R Got, G Azzar, R Letoublon, J Frot-Coutaz, M Nicol, V Azais-Braesco, G Pascal
Affiliations

Effect of vitamin A deficiency on the in vitro transfer of mannose and N-acetylglucosamine in rat liver nuclei

S Galland et al. Food Addit Contam. 1990.

Abstract

Liver nuclei, prepared from normal and vitamin A-deficient rats, were incubated in the presence of GDP-(14C)mannose or UDP-N-acetyl(14C)glucosamine and the labelled glycoproteins analysed by SDS PAGE. Fluorographic analysis has shown that (14C) mannose labelling is enhanced by vitamin A deficiency whereas N-acetyl(14C)glucosamine transfer remains approximately at the same level regardless of the vitamin A status; we did not notice any modification when the proteins were monitored by Coomassie blue or by silver nitrate.

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