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Comment
. 2012 May 25;110(11):1403-7.
doi: 10.1161/CIRCRESAHA.112.268961.

Biased DNA segregation during stem cell division

Piero Anversa  1 , Annarosa Leri, Jan Kajstura
Affiliations
Comment

Biased DNA segregation during stem cell division

Piero Anversa et al. Circ Res. .

Erratum in

  • Correction.
    [No authors listed] [No authors listed] Circ Res. 2015 Dec 4;117(12):e130. doi: 10.1161/RES.0000000000000085. Circ Res. 2015. PMID: 26635384 No abstract available.

Abstract

Adult skeletal muscle stem cells are a heterogeneous cell population characterized by a small subset of undifferentiated cells that express at high level the paired/homeodomain gene Pax7. This category of satellite cells divides predominantly by asymmetric chromatid segregation generating a daughter cell that carries the mother DNA and retains stem cell property, and a daughter cell that inherits the newly-synthesized DNA and acquires the myocyte lineage.

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Figures

Figure 1
Figure 1. Non-random and random chromatid segregation
With asymmetric chromatid segregation, a dividing mother stem cell synthesizes new DNA during S-phase and generates two daughter stem cells, one carrying only the mother DNA, which is the true stem cell, and the other only the newly-synthesized DNA. With symmetric chromatid segregation, a dividing stem cell synthesizes new DNA and generates two daughter stem cells, each carrying the mother DNA and the newly-synthesized DNA.
Figure 2
Figure 2. Cardiac stem cell niche
Five c-kit positive cells (green) surrounded by cardiomyocytes (myosin and desmin, red). One of the 5 c-kit-positive cells is brightly labeled by BrdU (yellow, arrow). A dimly labeled cardiomyocyte nucleus (arrowhead) is also present. The brightly BrdU-labeled c-kit-positive CSC may reflect a parent stem cell derived from a BrdU-negative grandparent stem cell that incorporated the halogenated nucleotide during the first division. Subsequently, the BrdU-labeled parent stem cell entered the G0/G1 phase. Adapted from reference .
Figure 3
Figure 3. c-myc and CSCs
Cardiac stem cell niche in a young rat heart contains eight c-kit-positive CSCs (green); two express c-myc (magenta, arrowheads). Nuclei are stained by DAPI. The attenuation in the expression of c-myc in CSCs with age is apparent by Western blotting. m, months. Tubulin, loading conditions.
Figure 4
Figure 4. Senescent human heart
The myocardium of a 79 years old man is characterized by cardiomyocytes (α-sarcomeric actin, α-SA, red) that express the senescence-associated protein p16INK4a (green, arrows).
See this image and copyright information in PMC

Comment on

References

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