Effect of intraperitoneal PERIDAN™ concentrate adhesion reduction device on clinical findings, infection, and tissue healing in an adult horse jejunojejunostomy model

Abstract

Objective: To evaluate the effect of PERIDAN™ Concentrate on clinical findings, infection, and tissue healing in adult horses undergoing celiotomy and jejunojejunostomy.

Study design: Block randomized blinded experimental in vivo study.

Animals: Adult horses (n = 12).

Methods: Horses had jejunojejunostomy at 2 sites and were administered 5 L of diluted PERIDAN™ Concentrate (6 horses) or Lactated Ringer's Injection (LRS) control intraperitoneally (6 horses) before body wall closure. Postoperative monitoring comprised physical examinations, serial hematology, coagulation and chemistry panels, and ultrasonographic examination. Horses were euthanatized 10 days postoperatively. Anastomoses and linea alba incisions were tested for mechanical strength; and tissue healing, inflammation, and infection were assessed by histological evaluation. Data were analyzed using a mixed model ANOVA. Level of significance was P < .05.

Results: No physical examination differences were observed between groups. Statistically significant differences were observed in leukocyte and neutrophil counts, prothrombin time, antithrombin III activity, intestinal bursting pressures, and histologic healing grade in the mid region of the linea alba. These differences were minimal, and of no observable clinical significance. Other blood variable and histologic differences between groups were not significant.

Conclusions: PERIDAN™ Concentrate was safely administered intraperitoneally to healthy horses undergoing celiotomy and anastomosis.