Flavobacterium enshiense sp. nov., isolated from soil, and emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense

Abstract

A Gram-negative, strictly aerobic, yellow-pigmented rod, designated DK69(T), was isolated from soil collected from the waste liquid treatment facility of Bafeng Pharmaceutical Company in the city of Enshi, Hubei Province, China. Phylogenetic analysis based on 16S rRNA gene sequences placed strain DK69(T) in the genus Flavobacterium of the family Flavobacteriaceae. The highest 16S rRNA gene sequence similarities were found with Flavobacterium cauense R2A-7(T) (96.9 %), Flavobacterium saliperosum AS 1.3801(T) (96.3 %) and Flavobacterium suncheonense GH29-5(T) (95.7 %). The major fatty acids (≥5 %) were iso-C15 : 0, iso-C17 : 1ω9c, C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The major respiratory quinone was menaquinone-6. The genomic DNA G+C content was 34.4 mol%. Strain DK69(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium enshiense sp. nov. is proposed. The type strain is DK69(T) ( = CCTCC AB 2011144(T) = KCTC 23775(T)). Emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense are also proposed.