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. 1990 Dec;87(24):9761-5.
doi: 10.1073/pnas.87.24.9761.

In vitro regulation of a SIN3-dependent DNA-binding activity by stimulatory and inhibitory factors

H Wang  1 , D J Stillman
Affiliations

In vitro regulation of a SIN3-dependent DNA-binding activity by stimulatory and inhibitory factors

H Wang et al. Proc Natl Acad Sci U S A. 1990 Dec.

Abstract

The yeast SIN3 gene (also known as SDII, is a known negative regulator of the yeast HO gene. A DNA-binding activity, called SDP1, which binds to the HO promoter, is absent in extracts prepared from sin3 mutants and has been proposed to function as a repressor. We show that SIN3 does not encode SDP1 and that SDP1 DNA-binding activity is modulated in vitro by two factors, an inhibitory factor, I-SDP1, and a stimulatory factor, S-SDP1. I-SDP1 acts as an in vitro inhibitor of the SDP1 DNA-binding activity. Restoration of the DNA-binding activity is achieved by inclusion of a stimulatory factor, S-SDP1, which copurifies with the SIN3 protein. SDP1 DNA-binding activity was restored by treating a protein fraction containing SDP1 and I-SDP1 with the dissociating agent formamide.

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