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. 2012 Oct;22(10):1422-5.
doi: 10.1038/cr.2012.84. Epub 2012 May 29.

Gametogenesis in a dish

Ying Gu  1 , Guang-Hui Liu, Juan Carlos Izpisua Belmonte
Affiliations

Gametogenesis in a dish

Ying Gu et al. Cell Res. 2012 Oct.

Abstract

Recent progress in the induced pluripotent stem cell (iPSC) field as well as the establishment of germline stem cell isolation and culture methodologies may provide an in vitro platform for the study of physiological and pathological human gamete development and open new avenues for cell replacement-based personalized treatment of infertility.

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Figures

Figure 1
Figure 1
Proposed model for in vitro gametogenesis from iPSCs or germline stem cells. The germline stem cells (SSCs from males, or OSC from females) could be isolated, cultured, and transplanted back to patients to restore fertility. Meanwhile, in vitro gametogenesis could be induced from both germline stem cells and patients-derived iPSCs to generate oocytes and sperm for both research and therapeutic purposes. Gene editing could be applied in cultured iPSCs, and germline stem cells to correct disease-related mutations. iPSCs: induced pluripotent stem cells. SSCs: spermatogonial stem cells. OSCs: oogonial stem cells.
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