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. 1990 Nov 15;272(1):45-9.
doi: 10.1042/bj2720045.

Antibody organization on lipid films. Influence of pH and interchain disulphide reduction

E E Uzgiris  1
Affiliations

Antibody organization on lipid films. Influence of pH and interchain disulphide reduction

E E Uzgiris. Biochem J. .

Abstract

Two distinct lattice structures are observed for two-dimensional (2-D) antibody organization on phospholipid films. A low-order, small-unit-cell, square lattice is obtained at pH 7 and below for mouse IgE, mouse IgG2a and IgG2b and rabbit IgG. At pH 7.5 and above, the observed lattice structure switches to a large-unit-cell, hexagonal type for rabbit IgG and mouse IgE. Interchain disulphide reduction by exposure to 2 mM-dithiothreitol results in the formation of the compact 2-D lattice for all cases and for all pH conditions.

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