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Comment
. 2012 Jul;4(7):541-2; author reply 543-4.
doi: 10.1002/emmm.201100203. Epub 2012 May 31.

RUNX3 is expressed in the epithelium of the gastrointestinal tract

Yoshiaki Ito
Comment

RUNX3 is expressed in the epithelium of the gastrointestinal tract

Yoshiaki Ito. EMBO Mol Med. 2012 Jul.
No abstract available

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Figures

Figure 1
Figure 1. Assortment of figures from earlier publications demonstrates the expression of Runx3/RUNX3 in mouse and human stomach epithelial cells

  1. In situ hybridization of mRunx3 is reproduced with permission from Fig 1I of Li et al (2002).

  2. IHC of human stomach epithelium using anti-RUNX3 monoclonal antibody is reproduced with permission from Ito et al (2005).

  3. In situ hybridization of hRUNX3 together with sense strand as control is reproduced with permission from Ito et al (2005).

Figure 2
Figure 2. Runx3 RNA extracted from purified GIT epithelium of adult mouse
RNA was extracted from indicated tissues. Reverse transcription was performed using equivalent RNA amounts. 1: wt stomach epi; 2: wt jejunum epi; 3: wt colon epi; 4: Runx3−/− stomach epi; 5: Runx3−/− jejunum epi; 6: Runx3−/− colon epi. Left, lanes 1–6 show PCR amplification of Runx3 transcript encompassing exon 4. Primers used are from exon 3 (mRx3-exon3-F; 5′-CGCTTCCGCTGTCATGAAG-3′) and exon 5 (mRx3-exon5-R; 5′-ATGCGCAGGTCTCCAAAG-3′). More than 70% of the Runx3 RNA in stomach is estimated to be from epithelial cells. PCR product amplified from Runx3−/− mice is shorter than that from WT mice. Sequencing of PCR product confirmed the absence of exon 4 in RNA samples obtained from Runx3−/− mice (unpublished observation). Right, lanes 1–6 show PCR products amplified using primers specific for exon 3 (mRx3-exon3-F) and LacZ gene (LacZ-R; 5′-CTCTTCGCTATTACGCCAGC-3′). As expected, no bands were amplified from WT samples. For Runx3−/− samples, LacZ containing bands were amplified in lanes 4–6. The presence of these bands indicates the presence of LacZ-containing transcripts, which is presumably too few to elicit blue staining in GIT epithelium. X 30 and X 35 refer to the number of PCR cycles. A 100 bp ladder marker was used (M).
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References

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