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. 2009 Jul;1(2):98-103.

Cell phenotypes in human amniotic fluid

D A Davydova  1 , E A Vorotelyak, Yu A Smirnova, R D Zinovieva, Yu A Romanov, N V Kabaeva, V V Terskikh, A V Vasiliev
Affiliations

Cell phenotypes in human amniotic fluid

D A Davydova et al. Acta Naturae. 2009 Jul.

Abstract

Stem cells capable of long-term proliferation and differentiation into different cell types may be a promising source of cells for regenerative medicine. Recently, much attention has been paid to fetal stem cells, among which are cells from amniotic fluid (AF). We have isolated amniotic stem cells from 3 AF samples. Flow cytometry, RT -PCR and immunohistochemistry have shown that these cells express mesenchymal (CD90, CD73, CD105, CD13, CD29, CD44, and CD146), neural (≤3-tubulin, Nestin, and Pax6), epithelial (keratin 19 and p63) markers and also markers of pluripotency (Oct4, Nanog, and Rex-1). Transplantation of the cells to nude mice does not lead to tumor formation. Thus, putative stem/progenitor cells from AF are capable of long-term proliferation in vitro and the profile of gene expression led us to speculate that they have greater differentiation potential than mesenchymal stem cells and may be useful for cell therapy.

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Figures

Fig. 1.
Fig. 1.
AF cells in culture. (a) Primary fibroblastic cell colony on day 9 of culture. (b) Primary epithelioid cell colony on day 9 of culture. (c) Cells grew to confluence in the 8th-passage culture. (a, b, c) Light field
Fig. 2.
Fig. 2.
The results of flow cytometry analyses of CD34, CD73, HLA-A,B,C and HLA-DR,DP,DQ expression
Fig. 3.
Fig. 3.
Immunohistochemical analyses of mesenchymal, neural and epithelial markers expression in cultured AF cells. Stained with antibodies against (a) CD105; (b) CD49d; (c) β3-tubulin; (d) keratin 19; (e) p63; (f) the same field, nuclei stained with DAPI. (a, b, c, d) Merge, nuclei stained with DAPI
Fig. 4.
Fig. 4.
Expression of differentiation and stem cell markers determined by RT-PCR
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