Comparative Study
Mitochondria contain a distinct DNA topoisomerase
- PMID: 226516
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Comparative Study
Mitochondria contain a distinct DNA topoisomerase
J Biol Chem.
.
Free article
Abstract
A topoisomerase (nicking-closing enzyme) has been isolated from rat liver mitochondria. It has purified by double-stranded DNA-cellulose chromatography approximately 50,000-fold, based on the crude mitochondrial extract. It possesses a minimum specific activity of 1.9 x 10(5) units/mg. The enzyme has been shown to be distinctly mitochondrial, differentiated from the nuclear topoisomerase by its sensitivity to the intercalating drug, ethidium bromide, and to the non-intercalating trypanocidal drug, Berenil.
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