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. 2011;8(4):467-76.
doi: 10.4314/ajtcam.v8i4.20. Epub 2011 Jun 1.

Effect of Shen-qi-di-huang decoction on reducing proteinuria by preserving nephrin in adriamycin-induced nephropathy rats

Hongyu Chen  1 , Qin Zhu, Xuanli Tang, Man Min, Leqin Jie, Lijing Chen
Affiliations

Effect of Shen-qi-di-huang decoction on reducing proteinuria by preserving nephrin in adriamycin-induced nephropathy rats

Hongyu Chen et al. Afr J Tradit Complement Altern Med. 2011.

Abstract

The aim of this study is to investigate the effect of Shen-qi-di-huang decoction on reducing proteinuria and to discuss the mechanism of its action in Adriamycin (ADR)-induced nephropathy rats. The rats were randomly divided into three groups (n=12 each group): normal control (group A); ADR model control (group B); ADR + Shen-qi-di-huang decoction (group C). In group B and C, the rats were intravenously injected with ADR (6.5mg/kg). The rats in group C were orally administrated with Shen-qi-di-huang decoction after the injection of ADR. On day 7, 14, 28, 56 after ADR injection, 24h urine protein was detected. On day 28, 56 after ADR injection, ALB, ALT, serum creatinine (Scr) and BUN were examined. The morphological changes of the kidneys were observed by light microscope and electron microscope on day 28, 56 after ADR injection. The expression of nephrin was determined by immunohistochemistry and RT-PCR on day 28, 56 after ADR injection. Compared with group B, 24h urine protein and Scr decreased in group C on day 56 (P<0.05). The expression of nephrin determined by immunohistochemistry and RT-PCR increased in group C on day 28, 56 (P<0.05). The morphology observed by light microscope and electron microscope improved in group C on day 28, 56. Shen-qi-di-huang decoction decreases proteinuria, protects kidney function, and ameliorates histopathology in ADR-induced rats by preserving nephrin expression.

Keywords: Shen-qi-di-huang decoction; adriamycin nephropathy; nephrin; proteinuria.

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Figures

Figure 1
Figure 1
24-h urinary protein(A), ALB(B), ALT(C), Scr(D), BUN(E) in rats of three groups on different time point. Normal control (group A); ADR model control (group B); ADR + Shen-qi-di-huang decoction (group C). *P<0.05 vs. group A, #P<0.05 vs. group B. (A: on day 7, 14, n=12 respectively; on day28, 56, n=6 respectively) (B, C, D, E: on day 28, 56, n=6 respectively)
Figure 2
Figure 2
Light microscopic findings in rats of three groups (H.E. 400×). Normal control (group A); ADR model control (group B); ADR + Shen-qi-di-huang decoction (group C). (A1: group A on day 28, B1: group B on day 28, C1: group C on day 28, A2: group A on day 56, B2: group B on day 56, C2: group C on day 56)
Figure 3
Figure 3
Transmission electron microscopic findings in rats of three groups (4000×). Normal control (group A); ADR model control (group B); ADR + Shen-qi-di-huang decoction (group C). (A1: group A on day 28, B1: group B on day 28, C1: group C on day 28, A2: group A on day 56, B2: group B on day 56, C2: group C on day 56)
Figure 4
Figure 4
Immunostaining of nephrin in rats of three groups (400×). Normal control (group A); ADR model control (group B); ADR + Shen-qi-di-huang decoction (group C).n=6 respectively. *P<0.05 vs. group A, #P<0.05 vs. group B. (A1: group A on day 28, B1: group B on day 28, C1: group C on day 28, A2: group A on day 56, B2: group B on day 56, C2: group C on day 56)
Figure 5
Figure 5
mRNA of nephrin in rats of three groups. Normal control (group A); ADR model control (group B); ADR + Shen-qi-di-huang decoction (group C).n=6 respectively. *P<0.05 vs. group A, #P<0.05 vs. group B. (A:GAPDH, B:nephrin day 28, C:nephrin day56, D: nephrin mRNA/ GAPDH mRNA in three groups on day 28,56)
Figure 6
Figure 6
The relationship of 24-h urinary protein, nephrin mRNA/GAPDH mRNA and nephrin-positive area /glomerulus area *100%.
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