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. 2012:2012:295802.
doi: 10.1100/2012/295802. Epub 2012 Apr 30.

Electrochemically pretreated carbon microfiber electrodes as sensitive HPLC-EC detectors

Zdenka Bartosova  1 , Daniel Riman, Petr Jakubec, Vladimir Halouzka, Jan Hrbac, David Jirovsky
Affiliations

Electrochemically pretreated carbon microfiber electrodes as sensitive HPLC-EC detectors

Zdenka Bartosova et al. ScientificWorldJournal. 2012.

Abstract

The paper focuses on the analysis and detection of electroactive compounds using high-performance liquid chromatography (HPLC) combined with electrochemical detection (EC). The fabrication and utilization of electrochemically treated carbon fiber microelectrodes (CFMs) as highly sensitive amperometric detectors in HPLC are described. The applied pretreatment procedure is beneficial for analytical characteristics of the sensor as demonstrated by analysis of the model set of phenolic acids. The combination of CFM with separation power of HPLC technique allows for improved detection limits due to unique electrochemical properties of carbon fibers. The CFM proved to be a promising tool for amperometric detection in liquid chromatography.

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Figures

Figure 1
Figure 1
Carbon fiber microelectrode, comparison to human hair.
Figure 2
Figure 2
Calibration curves for gallic (a) and caffeic acid (b) on pretreated and native carbon fiber electrodes. Inset: actual amperometric response curves determined in stirred mobile phase. Applied potential: 1200 mV (versus Ag/AgCl).
Figure 3
Figure 3
Scheme of the electrode arrangement used for HPLC measurements. Effluent flow direction is indicated by the arrow.
Figure 4
Figure 4
Hydrodynamic voltammograms of the phenolic acids used.
Figure 5
Figure 5
Chromatogram of standard mixture of phenolic acids. Conditions: amperometric detection at 1200 mV (versus Ag/AgCl), for other conditions see Section 2; analyte concentration: 10 ng/mL each. Inset chromatogram: analysis of 1 ng/mL concentration (2.5 pg on-column each acid). Legend: 1: gallic acid, 2: protocatechuic acid, 3: gentisic acid, 4: p-hydroxybenzoic acid, and 5: caffeic acid.
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