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. 2011 Summer;22(2):61-3.
doi: 10.1155/2011/802329.

A pilot survey of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae mediated by K pneumoniae serine carbapenemases in a regional referral hospital in British Columbia

Aubrey Shannon  1 , Ken Wagner, Naowarat Cheeptham, Gwen Stephens
Affiliations

A pilot survey of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae mediated by K pneumoniae serine carbapenemases in a regional referral hospital in British Columbia

Aubrey Shannon et al. Can J Infect Dis Med Microbiol. 2011 Summer.
No abstract available

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Figures

Figure 1)
Figure 1)
The number of resistance patterns observed in isolates of Escherichia coli and Klebsiella pneumoniae collected at Royal Inland Hospital in Kamloops, British Columbia (n=154). AMC Amoxicillin-clavulanic acid; CAZ Ceftazidime; CFP Cefoperazone; CIP Ciprofloxacin; CTX Cefotaxime; CZ Cefazolin; ETP Ertapenem; GM Gentamicin; MEM Meropenem; SXT Trimethoprim-sulfamethoxazole
Figure 2)
Figure 2)
Gel electrophoresis polymerase chain reaction products. Lane 1: Polymerase chain reaction reactants plus water, no DNA present; lane 2: 15 colonies of sample organisms; lane 3: One colony of Klebsiella pneumoniae ATCC BAA 1705 (K pneumoniae carbapenemase [KPC]-1-positive control [currently KPC-2, according to Yigit et al (19)]); lane 4: 15 colonies of known negative K pneumoniae ATCC BAA 1706 and one KPC-positive colony; lane 5: 1 Kb marker. Bands in lanes 3 and 4 represent amplified 399 bp fragments of blaKPC-1 from the KPC-1-positive control organism ATCC BAA 1705
Figure 3)
Figure 3)
Gel electrophoresis polymerase chain reaction products. Lane 1: 1 Kb marker; lane 2: One colony of Klebsiella pneumoniae ATCC BAA 1705 (K pneumoniae carbapenemase [KPC]-1-positive control [currently KPC-2, according to Yigit et al (19)]); lane 3: One colony of KPC-3-positive control organisms; lane 4: Negative control lane, polymerase chain reaction reactants plus water, no DNA present; lane 5: 1 Kb marker. Bands in lanes 2 and 3 represent amplified 399 bp fragments of KPC genes
Figure 4)
Figure 4)
Mass Klebsiella pneumoniae carbapenemase (KPC) gene screening of 154 patient isolates. Lane 1: K pneumoniae ATCC BAA 1705 (KPC-1-positive control [currently KPC-2, according to Yigit et al (19)]); lane 2: K pneumoniae KPC-2 positive; lane 3 to 14: Polymerase chain reaction products of mass preliminary screening (15 different patient isolates per lane); lane 15: Negative control. Bands observed in lanes 1 and 2 are expected 399 bp fragments from the blaKPC-1-positive and blaKPC-2-positive organisms
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References

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