Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 May 21:3:104.
doi: 10.3389/fpls.2012.00104. eCollection 2012.

Cotton fiber: a powerful single-cell model for cell wall and cellulose research

Candace H Haigler  1 , Lissete Betancur, Michael R Stiff, John R Tuttle
Affiliations

Cotton fiber: a powerful single-cell model for cell wall and cellulose research

Candace H Haigler et al. Front Plant Sci. .

Abstract

Cotton fibers are single-celled extensions of the seed epidermis. They can be isolated in pure form as they undergo staged differentiation including primary cell wall synthesis during elongation and nearly pure cellulose synthesis during secondary wall thickening. This combination of features supports clear interpretation of data about cell walls and cellulose synthesis in the context of high throughput modern experimental technologies. Prior contributions of cotton fiber to building fundamental knowledge about cell walls will be summarized and the dynamic changes in cell wall polymers throughout cotton fiber differentiation will be described. Recent successes in using stable cotton transformation to alter cotton fiber cell wall properties as well as cotton fiber quality will be discussed. Futurec prospects to perform experiments more rapidly through altering cotton fiberwall properties via virus-induced gene silencing will be evaluated.

Keywords: Gossypium; cellulose; cotton fiber; elongation; pectin; transition stage; virus-induced gene silencing; xyloglucan.

PubMed Disclaimer

Figures

FIGURE 1
FIGURE 1
A representation of the progression of cotton fiber development. The “stem” indicates the fastest developmental timeline when plants are grown under warm conditions. “Branches” show DPA with associated images of fiber. Cryo- field-emission SEM of (A) fiber initials on the ovule surface (bar = 10 μm); (B) twisting and elongating 3 DPA fibers (bar = 100 μm); (C) CFML stretched between two 3 DPA fibers (bar = 4 μm); (D) ordered bundles of fibers inside the boll (bar = 100 μm). Differential interference contrast micrographs indicating microfibril angle (as highlighted by arrows) in fiber at (E) 16 DPA and (F) 20 DPA (bars = 10 μm). TEM fiber cross-section show (G) an early stage of secondary wall thickening (bar = 300 μm); and (H) a more advanced stage of secondary wall deposition (bar = 1 μm). (I) Mature cotton boll and (J) cross-section of mature fiber viewed in the light microscope. SCW, secondary cell wall. Re-published with permission from The Cotton Foundation (http://www.cotton.org/foundation/index.cfm; Stiff and Haigler, in press).
See this image and copyright information in PMC

References

    1. Abidi N., Hequet E., Cabrales L. (2010). Changes in sugar composition and cellulose content during the secondary cell wall biogenesis in cotton fibers. Cellulose 17 153–160
    1. Ahn J., Verma R., Kim M., Lee J., Kim Y., Bang J., Reiter W., Pai H. (2006). Depletion of UDP-d-apiose/UDP-d-xylose synthases results in Rhamnogalacturonan-II deficiency, cell wall thickening, and cell death in higher plants. J. Biol. Chem. 281 13708–13716 - PubMed
    1. Al-Ghazi Y., Bourot S., Arioli T., Dennis E. S., Llewellyn D. J. (2009). Transcript profiling during fiber development identifies pathways in secondary metabolism and cell wall structure that may contribute to cotton fiber quality. Plant Cell Physiol. 50 1364–1381 - PubMed
    1. Amor Y., Haigler C. H., Johnson S., Wainscott M., Delmer D. P. (1995). A membrane-associated form of sucrose synthase and its potential role in synthesis of cellulose and callose in plants. Proc. Natl. Acad. Sci. U.S.A. 92 9353–9357 - PMC - PubMed
    1. An C., Saha S., Jenkins J. N., Scheffler B. E., Wilkins T. A., Stelly D. M. (2007). Transcriptome profiling, sequence characterization, and SNP-based chromosomal assignment of the EXPANSIN genes in cotton. Mol. Genet. Genomics 279 539–553 - PubMed

LinkOut - more resources