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. 2012 May 9;8(2):277-85.
doi: 10.5114/aoms.2012.28555.

Exhaled eicosanoids and biomarkers of oxidative stress in exacerbation of chronic obstructive pulmonary disease

Adam Antczak  1 , Maciej Ciebiada, Tadeusz Pietras, Wojciech J Piotrowski, Zofia Kurmanowska, Paweł Górski
Affiliations

Exhaled eicosanoids and biomarkers of oxidative stress in exacerbation of chronic obstructive pulmonary disease

Adam Antczak et al. Arch Med Sci. .

Abstract

Introduction: Eicosanoids and oxidants play an important role in inflammation, but their role in chronic obstructive pulmonary disease (COPD) is uncertain. In this study we hypothesized that levels of exhaled leukotrienes, prostaglandins and biomarkers of oxidative stress are increased in infectious exacerbations of COPD and that they decrease after antibiotic therapy.

Material and methods: Cysteinyl-leukotrienes (LTs), leukotriene B(4) (LTB(4)), prostaglandin E(4), hydrogen peroxide (H(2)O(2)) and 8-isoprostane were measured in exhaled breath condensate (EBC) in 16 COPD patients with infectious exacerbations (mean age 64 ±12 years, 13 male) on day 1, during antibiotic therapy (days 2-4), 2-4 days after therapy and at a follow-up visit when stable (21-28 days after therapy).

Results: There was a significant fall in concentration of cys-LTs, LTB(4) and 8-isoprostane at visit 3 compared to day 1 (cys-LTs: 196.5 ±38.4 pg/ml vs. 50.1 ±8.2 pg/ml, p < 0.002; LTB(4): 153.6 ±25.5 pg/ml vs. 71.9 ±11.3 pg/ml, p < 0.05; 8-isoprostane: 121.4 ±14.6 pg/ml vs. 56.1 ±5.2 pg/ml, p < 0.03, respectively). Exhaled H(2)O(2) was higher on day 1 compared to that at visits 2 and 3 (0.74 ±0.046 µM vs. 0.52 ±0.028 µM and 0.35 ±0.029 µM, p < 0.01 and p < 0.01, respectively). Exhaled PGE(2) levels did not change during exacerbations of COPD. Exhaled eicosanoids and H(2)O(2) in EBC measured at the follow-up visit (stable COPD) were significantly higher compared to those from healthy subjects.

Conclusions: We conclude that eicosanoids and oxidants are increased in infectious exacerbations of COPD. They are also elevated in the airways of stable COPD patients compared to healthy subjects.

Keywords: 8-isoprostane; chronic obstructive pulmonary disease; exhaled breath condensate; exhaled eicosanoids.

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Figures

Figure 1
Figure 1
Cys-LTs in expired breath condensate of exacerbated COPD patients
Figure 2
Figure 2
The LTB4 in expired breath condensate of exa-cerbated COPD patients
Figure 3
Figure 3
The PGE2 in expired breath condensate of exa-cerbated COPD patients
Figure 4
Figure 4
The 8-isoprostane levels in expired breath condensate of exacerbated COPD patients
Figure 5
Figure 5
The H2O2 in expired breath condensate of exa cerbated COPD patients
Figure 6
Figure 6
Positive correlation between 8-isoprostane and H2O2 levels in expired breath condensate of exa - cerbated COPD patients at visit 1 (day 1)
Figure 7
Figure 7
Changes of FEV1 (A) and pO2 (B) in patients with exacerbation of COPD
See this image and copyright information in PMC

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