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. 2012 Jun;86(6):1039-42.
doi: 10.4269/ajtmh.2012.11-0454.

Use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis for rapid confirmation of Burkholderia pseudomallei in septicemic melioidosis

Timothy J J Inglis  1 , Paul E Healy, Leith J Fremlin, Clayton L Golledge
Affiliations

Use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis for rapid confirmation of Burkholderia pseudomallei in septicemic melioidosis

Timothy J J Inglis et al. Am J Trop Med Hyg. 2012 Jun.

Abstract

Burkholderia pseudomallei was quickly identified from blood cultures collected from septicemic patients by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis using an in-house reference library. This procedure reduced the time to definitive identification by more than 24 hours. This analysis is a useful addition to laboratory methods for early recognition of septicemic melioidosis in non-endemic settings.

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Conflict of interest statement

Disclosure: Leith J. Fremlin is an employee of Bruker Biosciences, the manufacturers of the MALDI-TOF MS we used. His role in this work was to assist in the bioinformatic software analysis of MS results and reporting of that process as a scientist. This statement is made in the interest of full disclosure and not because the authors consider this to be a conflict of interest.

Figures

Figure 1.
Figure 1.
Burkholderia reference strains analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and displayed in dendrogram format by using proprietary software (MALDI Biotyper; Bruker Daltonics, Bremen, Germany). Burkholderia pseudomallei NCTC 13177, DM 98, and BCC 215, B. thailandensis Bt4 (BT4), and B. ubonensis Bu (BU) have been fully sequenced. These and B. pseudomallei NCTC 10276 are archived in the Western Australian Burkholderia Collection reference collection (WABCr). 1d, 2d, 3d, and 7d indicate isolates grown on 5% horse blood agar for 1, 2, 3, days and for 24 hours, then held at ambient room temperature for 6 days (7d) ASA = Ashdown's selective agar; BPSA = B. pseudomallei selective agar; MSP = mass spectrophotometer pattern simi.
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