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. 2012:2012:310745.
doi: 10.1100/2012/310745. Epub 2012 Apr 30.

Combined effect of subchondral drilling and hyaluronic acid with/without diacerein in full-thickness articular cartilage lesion in rabbits

Wanwisa Suwannaloet  1 , Wiroon Laupattarakasem, Peerapol Sukon, Siriwan Ong-Chai, Pisamai Laupattarakasem
Affiliations

Combined effect of subchondral drilling and hyaluronic acid with/without diacerein in full-thickness articular cartilage lesion in rabbits

Wanwisa Suwannaloet et al. ScientificWorldJournal. 2012.

Abstract

The osteochondral healing potential of hyaluronic acid (HA) plus diacerein was evaluated in subchondral-drilling- (SCD-) induced fibrocartilage generation in rabbits. A full-thickness chondral defect was created along the patellar groove of both knees and then SCD was subsequently performed only in the left knee. A week later, the rabbits were allocated into 3 groups to receive weekly intra-articular (IA) injection for 5 weeks with normal saline solution (NSS) (group 1) or with HA (group 2 and group 3). Starting at the first IA injection, rabbits were also gavaged daily for 9 weeks with NSS (group 1 and group 2) or with diacerein (group 3). The animals were then sacrificed for evaluation. The newly formed tissue in SCD lesions showed significantly better histological grading scale and had higher content of type II collagen in HA-treated group compared to NSS control. In addition, adding oral diacerein to HA injection enhanced healing potential of HA.

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Figures

Figure 1
Figure 1
Gross morphology, H&E-stained, and immunohistochemical stained sections of normal (left panel), FTD (middle panel), and FTD plus SCD (right panel) 10 weeks after operation (Col I: collagen I; Col II: collagen II; NSS: normal saline solution; HA: hyaluronic acid; DC: diacerein).
Figure 2
Figure 2
The histological sections of femoral condyle stained with H&E 10 weeks after operation. The sections showed the repaired tissue treated with HA + NSS (c, d) and HA + diacerein (e, f) compared with the control (a, b) (NSS: normal saline solution; HA: hyaluronic acid; DC: diacerein).
Figure 3
Figure 3
Effect of HA + NSS and HA + DC on ICRS scores for the cartilaginous tissue regeneration of FTD (white bar) and SCD (black bar) 10 weeks after operation. Each value represents the mean ± SEM, n = 10. *P < 0.05, **P < 0.001 significantly different from control; # P < 0.05 significant difference between two groups. (NSS: normal saline solution; HA: hyaluronic acid; DC: diacerein; ICRS: International Cartilage Repair Society; SCD: subchondral drilling; FTD: full-thickness articular defects).
Figure 4
Figure 4
The mean score of the sections of repair cartilage varied according to morphology. Bars represent standard error of the mean (SEM) (NSS: normal saline solution; HA: hyaluronic acid; DC: diacerein; SCD: subchondral drilling; FTD: full-thickness articular defects).
Figure 5
Figure 5
Effect of HA + NSS and HA + DC on cell proliferation, using PCNA staining, 10 weeks after operation. Each value represents the mean ± SEM, n = 10. *P < 0.05, **P < 0.001 significantly different from control; # P < 0.05 significant difference between two groups (NSS: normal saline solution; HA: hyaluronic acid; DC: diacerein; SCD: subchondral drilling; FTD: full-thickness articular defects).
Figure 6
Figure 6
Effect of HA + NSS and HA + DC on type I collagen 10 weeks after operation. Each value represents the mean ± SEM, n = 10 (NSS: normal saline solution; HA: hyaluronic acid; DC: diacerein; SCD: subchondral drilling; FTD: full-thickness articular defects).
Figure 7
Figure 7
Effect of HA + NSS and HA + DC on type II collagen 10 weeks after operation. Each value represents the mean ± SEM, n = 10. *P < 0.05, **P < 0.001 significantly different from control; # P < 0.05 significant difference between two groups (NSS: normal saline solution; HA: hyaluronic acid; DC: diacerein; SCD: subchondral drilling; FTD: full-thickness articular defects).
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