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. 2012:2012:542937.
doi: 10.1100/2012/542937. Epub 2012 May 1.

PHBV/PCL microparticles for controlled release of resveratrol: physicochemical characterization, antioxidant potential, and effect on hemolysis of human erythrocytes

Jessica Bitencourt Emilio Mendes  1 , Manoela Klüppel Riekes, Viviane Matoso de Oliveira, Milton Domingos Michel, Hellen Karine Stulzer, Najeh Maissar Khalil, Sônia Faria Zawadzki, Rubiana Mara Mainardes, Paulo Vitor Farago
Affiliations

PHBV/PCL microparticles for controlled release of resveratrol: physicochemical characterization, antioxidant potential, and effect on hemolysis of human erythrocytes

Jessica Bitencourt Emilio Mendes et al. ScientificWorldJournal. 2012.

Abstract

Microparticles of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(ε-caprolactone) (PCL) containing resveratrol were successfully prepared by simple emulsion/solvent evaporation. All formulations showed suitable encapsulation efficiency values higher than 80%. PHBV microparticles revealed spherical shape with rough surface and presence of pores. PCL microparticles were spherically shaped with smooth surface. Fourier-transformed infrared spectra demonstrated no chemical bond between resveratrol and polymers. X-ray powder diffraction patterns and differential scanning calorimetry analyses indicated that microencapsulation led to drug amorphization. These PHBV/PCL microparticles delayed the dissolution profile of resveratrol. Release profiles were better fitted to biexponential equation. The hypochlorous-acid-scavenging activity and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation discoloration assay confirmed that the antioxidant activity of PHBV/PCL microparticles was kept, but was dependent on the microparticle morphology and dissolution profile. Resveratrol-loaded PHBV/PCL microparticles showed no cytotoxic effect on red blood cells.

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Figures

Figure 1
Figure 1
Chemical structure of resveratrol.
Figure 2
Figure 2
Scanning electron micrographs of PHBV/PCL microparticles: M1R0 (a), M1R5 (b), M1R10 (c), M1R20 (d), M2R0 (e), M2R5 (f), M2R10 (g), and M2R20 (h). Magnifications of 2000x.
Figure 3
Figure 3
FTIR spectra of resveratrol, PHBV/PCL, physical mixtures, and PHBV/PCL microparticles.
Figure 4
Figure 4
XRPD patterns of resveratrol, PHBV/PCL, physical mixtures, and PHBV/PCL microparticles.
Figure 5
Figure 5
TG and DTG curves of resveratrol.
Figure 6
Figure 6
TG curves of resveratrol, PHBV/PCL and physical mixtures (a) and (b), PHBV microparticles (c), and PCL microparticles (d).
Figure 7
Figure 7
DSC curves of resveratrol, PHBV/PCL, physical mixtures, and PHBV/PCL microparticles.
Figure 8
Figure 8
In vitro release profiles of pure drug and resveratrol-loaded PHBV/PCL microparticles.
Figure 9
Figure 9
Antioxidant effect of pure resveratrol, and PHBV/PCL microparticles by HOCl- (a) and ABTS∙+- (b) scavenging activity.
Figure 10
Figure 10
Absorbance values resulting from the released hemoglobin in erythrocyte hemolysis assay for blank, pure resveratrol, and PHBV/PCL microparticles. The asterisk indicates statistically significant difference (P = 0.0467).
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References

    1. Karacay Ö, Sepici-Dincel A, Karcaaltincaba D, et al. A quantitative evaluation of total antioxidant status and oxidative stress markers in preeclampsia and gestational diabetic patients in 24–36 weeks of gestation. Diabetes Research and Clinical Practice. 2010;89(3):231–238. - PubMed
    1. Pandey KB, Rizvi SI. Resveratrol may protect plasma proteins from oxidation under conditions of oxidative stress in vitro. Journal of the Brazilian Chemical Society. 2010;21(5):909–913.
    1. Premkumar K, Bowlus CL. Ascorbic acid reduces the frequency of iron induced micronuclei in bone marrow cells of mice. Mutation Research—Genetic Toxicology and Environmental Mutagenesis. 2003;542(1-2):99–103. - PubMed
    1. Barreiros ALBS, David JM, David JP. Estresse oxidativo: relação entre geração de espécies reativas e defesa do organismo. Quimica Nova. 2006;29(1):113–123.
    1. Shi G, Rao L, Yu H, Xiang H, Yang H, Ji R. Stabilization and encapsulation of photosensitive resveratrol within yeast cell. International Journal of Pharmaceutics. 2008;349(1-2):83–93. - PubMed