Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2012 May 30;9(6):555-66.
doi: 10.1038/nmeth.2015.

Selected reaction monitoring-based proteomics: workflows, potential, pitfalls and future directions

Paola Picotti  1 , Ruedi Aebersold
Affiliations
Review

Selected reaction monitoring-based proteomics: workflows, potential, pitfalls and future directions

Paola Picotti et al. Nat Methods. .

Abstract

Selected reaction monitoring (SRM) is a targeted mass spectrometry technique that is emerging in the field of proteomics as a complement to untargeted shotgun methods. SRM is particularly useful when predetermined sets of proteins, such as those constituting cellular networks or sets of candidate biomarkers, need to be measured across multiple samples in a consistent, reproducible and quantitatively precise manner. Here we describe how SRM is applied in proteomics, review recent advances, present selected applications and provide a perspective on the future of this powerful technology.

PubMed Disclaimer

References

    1. J Proteome Res. 2010 Feb 5;9(2):761-76 - PubMed
    1. Mol Cell Proteomics. 2005 Oct;4(10):1614-25 - PubMed
    1. Proteomics. 2010 May;10(10):2050-6 - PubMed
    1. Anal Chem. 2009 Sep 15;81(18):7823-8 - PubMed
    1. Rapid Commun Mass Spectrom. 2004;18(4):491-8 - PubMed

Publication types

MeSH terms

LinkOut - more resources