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. 2012 May 30;9(6):567-74.
doi: 10.1038/nmeth.2043.

Counting stem cells: methodological constraints

Leonid V Bystrykh  1 , Evgenia Verovskaya, Erik Zwart, Mathilde Broekhuis, Gerald de Haan
Affiliations

Counting stem cells: methodological constraints

Leonid V Bystrykh et al. Nat Methods. .

Abstract

The number of stem cells contributing to hematopoiesis has been a matter of debate. Many studies use retroviral tagging of stem cells to measure clonal contribution. Here we argue that methodological factors can impact such clonal analyses. Whereas early studies had low resolution, leading to underestimation, recent methods may result in an overestimation of stem-cell counts. We discuss how restriction enzyme choice, PCR bias, high-throughput sequencing depth and tagging method could affect the conclusions of clonal studies.

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References

    1. Methods. 2009 Apr;47(4):261-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4564-8 - PubMed
    1. Blood. 2010 Jun 3;115(22):4356-66 - PubMed
    1. Nat Med. 2009 Dec;15(12):1431-6 - PubMed
    1. Cell. 1985 Aug;42(1):71-9 - PubMed

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