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. 2012:889:385-406.
doi: 10.1007/978-1-61779-867-2_24.

DNA methylation screening and analysis

Karilyn E Sant  1 , Muna S Nahar, Dana C Dolinoy
Affiliations

DNA methylation screening and analysis

Karilyn E Sant et al. Methods Mol Biol. 2012.

Abstract

DNA methylation is an epigenetic form of gene regulation that is universally important throughout the life course, especially during in utero and postnatal development. DNA methylation aids in cell cycle regulation and cellular differentiation processes. Previous studies have demonstrated that DNA methylation profiles may be altered by diet and the environment, and that these profiles are especially vulnerable during development. Thus, it is important to understand the role of DNA methylation in developmental governance and subsequent disease progression. A variety of molecular methods exist to assay for global, gene-specific, and epigenome-wide methylation. Here we describe these methods and discuss their relative strengths and limitations.

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Figures

Fig. 1
Fig. 1
Example of a pyrogram produced during LUMA. The ratio of peaks provides methylation percentage.
See this image and copyright information in PMC

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