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. 2012 May 15;111(10):102810-1028107.
doi: 10.1063/1.4714727. Epub 2012 May 17.

Combinatorial growth of oxide nanoscaffolds and its influence in osteoblast cell adhesion

Claudia Y Acevedo-Morantes, Roberto A Irizarry-Ortiz, Pablo G Caceres-Valencia, Surinder P Singh, Jaime E Ramirez-Vick

Combinatorial growth of oxide nanoscaffolds and its influence in osteoblast cell adhesion

Claudia Y Acevedo-Morantes et al. J Appl Phys. .

Abstract

We report a novel method for high-throughput investigations on cell-material interactions based on metal oxide nanoscaffolds. These scaffolds possess a continuous gradient of various titanium alloys allowing the compositional and morphological variation that could substantially improve the formation of an osseointegrative interface with bone. The model nanoscaffold has been fabricated on commercially pure titanium (cp-Ti) substrate with a compositional gradients of tin (Sn), chromium (Cr), and niobium (Nb) deposited using a combinatorial approach followed by annealing to create native oxide surface. As an invitro test system, the human fetal osteoblastic cell line (hFOB 1.19) has been used. Cell-adhesion of hFOB 1.19 cells and the suitability of these alloys have been evaluated for cell-morphology, cell-number, and protein adsorption. Although, cell-morphology was not affected by surface composition, cell-proliferation rates varied significantly with surface metal oxide composition; with the Sn- and Nb-rich regions showing the highest proliferation rate and the Cr-rich regions presenting the lowest. The results suggest that Sn and Nb rich regions on surface seems to promote hFOB 1.19 cell proliferation and may therefore be considered as implant material candidates that deserve further analysis.

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Figures

Figure 1
Figure 1
Localization of the 28 points in the triangle inside of the CCS disc and the nine points on the cpTi controls (points A-I).
Figure 2
Figure 2
SEM of surface topography of the CSS disc in the points: (a) point 1 Sn-rich area, (b) point 7 Cr-rich-area, (c) point 13 Nb-rich area, and (d) center point 24. Pictures show variations in the surface topography of the substrate as well as variation in size of the titanium oxide scaffolds.
Figure 3
Figure 3
CLSM images of F-actin cytoskeleton (Phalloidin-TRITC labeled, red) and nuclei (DAPI labeled, blue) in hFOB 1.19 cells, growing on CCS disc after 3 days of culture. (a) Point 1; (b) Point 13; (c) Point 7; (d) center area; and (e) Ti6Al4V disc used as positive control group for cell-proliferation. Magnification: 10X.
Figure 4
Figure 4
Contour maps showing cell proliferation after 7, 14, and 21 days of culture.
See this image and copyright information in PMC

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