High quality draft genome sequence of Segniliparus rugosus CDC 945(T)= (ATCC BAA-974(T))

Abstract

Segniliparus rugosus represents one of two species in the genus Segniliparus, the sole genus in the family Segniliparaceae. A unique and interesting feature of this family is the presence of extremely long carbon-chain length mycolic acids bound in the cell wall. S. rugosus is also a medically important species because it is an opportunistic pathogen associated with mammalian lung disease. This report represents the second species in the genus to have its genome sequenced. The 3,567,567 bp long genome with 3,516 protein-coding and 49 RNA genes is part of the NIH Roadmap for Medical Research, Human Microbiome Project.

Keywords: Human Microbiome Project; Segniliparaceae,genome sequencing.

Figure1

Scanning electron micrograph of S. rugosus CDC 945^T. The scale bar is 667nm.

Figure 2

Maximum likelihood phylogenetic tree was generated using PHYML v2.2.4 [7] based on 16S rRNA sequences highlighting the position of S. rugosus CDC 945^T relative to the other type strains of mycolic acid containing genera in the suborder Corynebacterineae. GenBank accession numbers are listed after the name. The tree was inferred from 1,468 bp positions aligned using Clustal W [8] in MEGA v4 [9]. Numbers at the branch nodes are support values from 1,000 bootstrap replicates if equal to or greater than 70%. The scale bar indicates substitutions per site. The tree was rooted with Streptomyces coelicolor. Lineages with type strain genome sequencing projects registered in GOLD [10] are shown in blue, published genomes in bold.

Figure 3

Graphical circular map of the genome. From outside to the center: Genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), GC content, GC skew.