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Randomized Controlled Trial
. 2012 Jul;10(3):368-76.
doi: 10.2450/2012.0095-11. Epub 2012 May 4.

Genetic polymorphisms of human platelet antigens-1 to -6, and -15 in the Malaysian population

Jia-Yi Tan  1 , Lay-Hoong Lian, Veera Sekaran Nadarajan
Affiliations
Randomized Controlled Trial

Genetic polymorphisms of human platelet antigens-1 to -6, and -15 in the Malaysian population

Jia-Yi Tan et al. Blood Transfus. 2012 Jul.

Abstract

Background: Human platelet antigens (HPA) are determinant in several platelet-specific alloimmune disorders, such as neonatal alloimmune thrombocytopenia, post-transfusion purpura and platelet transfusion refractoriness. The distribution of HPA systems in the Malaysian population is not known. Defining the patterns of HPA systems provides a basis for risk assessment and management of the above complications.

Materials and methods: The aim of this study was to investigate the distribution of HPA -1 to -6 and -15 in the three major ethnic groups (Malay, Chinese and Indian) in the Malaysian population. A total of 600 random donor samples, 200 from each of the three ethnic groups, were genotyped by means of real time polymerase chain reaction (PCR) with hydrolysis probes and PCR-restriction fragment length polymorphism (PCR-RFLP).

Results: The most common genotype observed in this study was HPA-1a/1a-2a/2a-3a/3b-4a/4a-5a/5a-6a/6a-15a/15b (17%) followed by HPA-1a/1a-2a/2a-3a/3a-4a/4a-5a/5a-6a/6a-15a/15b (14.33%). The allele frequencies of HPA in Malays and Chinese were found to be similar those of other East and South-East Asian populations, while those of Indians were comparable to the frequencies found in Europeans.

Conclusion: The results of this study have been useful for determining the distribution of HPA polymorphisms in this region and for potential clinical implications.

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Figures

Figure 1
Figure 1
Heat-map showing gene frequencies for the “a” allele of HPA-1 to -6 and -15, together with a cluster dendrogram showing the relationships of various racial groups based on the frequency distribution.
Figure 2
Figure 2
Principal component analysis based on the gene frequencies of HPA-1 to -5 and -15 showing the relationships among various racial groups worldwide. Contributions of the first and second components were 69.76% and 16.95%, respectively.
See this image and copyright information in PMC

References

    1. Rozman P. Platelet antigens. The role of human platelet alloantigens (HPA) in blood transfusion and transplantation. Transpl Immunol. 2002;10:165–81. - PubMed
    1. Ghevaert C, Rankin A, Huiskes E, et al. Alloantibodies against low-frequency human platelet antigens do not account for a significant proportion of cases of fetomaternal alloimmune thrombocytopenia: evidence from 1054 cases. Transfusion. 2009;49:2084–9. - PubMed
    1. McBride SE. Real-time PCR assays for high-throughput human platelet antigen typing. In: Bugert P, editor. DNA and RNA Profiling in Human Blood Methods in Molecular Biology. New York: Humana Press; 2009. pp. 39–49. - PubMed
    1. Curtis BR. Genotyping for human platelet alloantigen polymorphisms: applications in the diagnosis of alloimmune platelet disorders. Semin Thromb Hemost. 2008;34:539–48. - PubMed
    1. Kulkarni B, Mohanty D, Ghosh K. Frequency distribution of human platelet antigens in the Indian population. Transfus Med. 2005;15:119–24. - PubMed

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