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. 2012 Jan;3(1):48-51.
doi: 10.4103/0976-9668.95949.

Effect of novel phosphoramidate on growth and respiratory metabolism of Paramecium aurelia

Benbouzid Houneida  1 , H Berrebah, M Berredjem, M R Djebar
Affiliations

Effect of novel phosphoramidate on growth and respiratory metabolism of Paramecium aurelia

Benbouzid Houneida et al. J Nat Sci Biol Med. 2012 Jan.

Abstract

The continuous increase in the number of new chemicals as well as the discharges of solid and liquid wastes triggered the need for simple and inexpensive bioassays for routine testing. In recent years, there has been increasing development of methods (particularly rapid tests) for testing environmental samples. This paper describes the quick toxic evaluation of a novel synthetic compound: Phosphoramidate derivative B at different concentrations (2, 4 and 8 μM) for 72 h on Paramecium aurelia. We showed that B concentrations affect the growth of Paramecium in concentration- dependent manner; also it decreases the growth rate and increases response percentage in concentration- dependent manner. The value of LC50 obtained for these protozoa was estimated at 4.9693 μM after 24 hours of exposure. The respiratory metabolism of protozoan is perturbed at three concentrations, noting that the oxygen consumption was significantly increased at high concentrations after 18 hours of exposure. The results indicate that the Paramecium toxicity assay could be used as a complementary system to rapidly elucidate the cytotoxic potential of insecticides. The major advantages associated with these tests are: inexpensive, simple, rapid and seem to be attractive alternatives to conventional bioassays.

Keywords: Cytotoxic tests; Paramecium aurelia; growth kinetics; phosphoramidate B; respiratory metabolism.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Total number of paramecia cells with the B concentrations assayed. Each data point represents the mean of three independent assays ± standard error. Values are signifi cant from control values at P < 0.001
Figure 2
Figure 2
Evolution of the response percentage of paramecia in presence of different concentrations of B. Each value is mean ± standard error of three replicates
Figure 3
Figure 3
Impact of B (2, 6 and 8 μM) on respiratory metabolism of Paramecium aurelia. Each value is mean ± standard error of three independent observations
See this image and copyright information in PMC

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