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Comparative Study
. 2012 Aug;50(8):2810-3.
doi: 10.1128/JCM.01412-12. Epub 2012 Jun 12.

Is real-time PCR better than conventional PCR for Mycobacterium tuberculosis complex detection in clinical samples?

Enrico Tortoli  1 , Pasquale Urbano, Fiorella Marcelli, Tullia M Simonetti, Daniela M Cirillo
Affiliations
Comparative Study

Is real-time PCR better than conventional PCR for Mycobacterium tuberculosis complex detection in clinical samples?

Enrico Tortoli et al. J Clin Microbiol. 2012 Aug.

Abstract

Cobas Amplicor MTB and later Cobas TaqMan MTB were used to test a very large series of consecutive specimens received for tuberculosis diagnosis. Performance parameters were estimated and compared overall and for separate specimen categories. Both systems showed excellent specificity, and that of TaqMan was the higher. The sensitivities were similar but satisfactory only with respiratory specimens and smear-positive samples.

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