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Comparative Study
. 2012 Aug;19(8):1326-8.
doi: 10.1128/CVI.00060-12. Epub 2012 Jun 13.

Comparison of mother and child antibodies that target high-molecular-mass Toxoplasma gondii antigens by immunoblotting improves neonatal diagnosis of congenital toxoplasmosis

Coralie L'Ollivier  1 , Martine Wallon, Benoit Faucher, Renaud Piarroux, François Peyron, Jacqueline Franck
Affiliations
Comparative Study

Comparison of mother and child antibodies that target high-molecular-mass Toxoplasma gondii antigens by immunoblotting improves neonatal diagnosis of congenital toxoplasmosis

Coralie L'Ollivier et al. Clin Vaccine Immunol. 2012 Aug.

Abstract

This retrospective study proposes a new reading of immunoblotting (IB) in the diagnosis of congenital toxoplasmosis. Our findings demonstrate that a three-IgM-band association at 75, 90, and 100 kDa called the IgM triplet increases the sensitivity to 95.8% when combined with prenatal and serological neonatal tests.

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Figures

Fig 1
Fig 1
IgM immunoblot analysis with paired mother and newborn sera from an infant congenitally infected with Toxoplasma gondii. Arrows show the IgM triplet defined by the association of 3 equidistant bands at 75, 90, and 100 kDa. (Left panel) The 90- and 100-kDa bands in the blot of the infant's serum were not present in the corresponding blot of the mother's serum. These supplementary bands were associated with the 75-kDa band in the IgM triplet. Other supplementary bands are present in low- and medium-molecular-mass bands in the blot of this infant's serum. (Right panel) Higher-magnification image from blot on the left highlighting the IgM triplet. m, mother; i, infant.
See this image and copyright information in PMC

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