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. 1979 Aug;76(8):3755-9.
doi: 10.1073/pnas.76.8.3755.

Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12

F Colbere-Garapin, S Chousterman, F Horodniceanu, P Kourilsky, A C Garapin

Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12

F Colbere-Garapin et al. Proc Natl Acad Sci U S A. 1979 Aug.

Abstract

A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned in Escherichia coli. A recombinat plasmid, pFG5, has been analyzed extensively and a detailed restriction map is presented. pFG5 DNA efficiently transforms TK- mouse L cells. The TK coding sequence in the cloned fragment has been localized and a smaller recombinant plasmid, pAG0, also carrying an active TK gene, has been constructed to serve as a more convenient vector for transfer, into TK- cells, of genes previously cloned in E. coli.

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